This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


Thermolysin

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 2: Line 2:
[[Thermolysin]] (TML) is a thermostable metalloproteinase enzyme from ''Bacillus thermoproteolyticus''. It catalyzes the hydrolysis of peptide bonds containing hydrophobic residues. Thermolysin is a well researched [[metalloproteases|metalloprotease]] containing <scene name='User:Ralf_Stephan/Sandbox_2/Zinc/2'>zinc</scene> (click this!) and the amino acids His-Glu-X-His-His as its catalytic center. <scene name='User:Ralf_Stephan/Sandbox_2/Res_yellow/3'>Glu-166, His-142 and -146 are grouped around the zinc atom</scene>, holding it fast, while <scene name='User:Ralf_Stephan/Sandbox_2/Res/1'>Glu-143 holds the polarized water atom. Additionally, Tyr-157 and His-231</scene> stabilize the substrate protein which will be cleaved into two smaller proteins.<ref>Matthews, BW. (1988): ''Structural basis of the action of thermolysin and related zinc peptidases''. In: ''Acc. Chem. Res.'' '''21'''(9); 333–340; http://dx.doi.org/10.1021/ar00153a003</ref><ref>PMID:11935352</ref>. See [[Metalloproteases]] and [[Matrix metalloproteinase]] for discussion.
[[Thermolysin]] (TML) is a thermostable metalloproteinase enzyme from ''Bacillus thermoproteolyticus''. It catalyzes the hydrolysis of peptide bonds containing hydrophobic residues. Thermolysin is a well researched [[metalloproteases|metalloprotease]] containing <scene name='User:Ralf_Stephan/Sandbox_2/Zinc/2'>zinc</scene> (click this!) and the amino acids His-Glu-X-His-His as its catalytic center. <scene name='User:Ralf_Stephan/Sandbox_2/Res_yellow/3'>Glu-166, His-142 and -146 are grouped around the zinc atom</scene>, holding it fast, while <scene name='User:Ralf_Stephan/Sandbox_2/Res/1'>Glu-143 holds the polarized water atom. Additionally, Tyr-157 and His-231</scene> stabilize the substrate protein which will be cleaved into two smaller proteins.<ref>Matthews, BW. (1988): ''Structural basis of the action of thermolysin and related zinc peptidases''. In: ''Acc. Chem. Res.'' '''21'''(9); 333–340; http://dx.doi.org/10.1021/ar00153a003</ref><ref>PMID:11935352</ref>. See [[Metalloproteases]] and [[Matrix metalloproteinase]] for discussion.
 +
</StructureSection>
== 3D Structures of Thermolysin ==
== 3D Structures of Thermolysin ==
Updated on {{REVISIONDAY2}}-{{MONTHNAME|{{REVISIONMONTH}}}}-{{REVISIONYEAR}}
Updated on {{REVISIONDAY2}}-{{MONTHNAME|{{REVISIONMONTH}}}}-{{REVISIONYEAR}}
 +
{{#tree:id=OrganizedByTopic|openlevels=0|
-
=== Thermolysin ===
+
* Thermolysin
-
[[2whz]], [[3fvp]], [[3dnz]], [[3do0]], [[3do1]], [[3do2]], [[2g4z]], [[2a7g]], [[1gxw]], [[1kei]], [[1l3f]], [[1tlx]], [[2tlx]], [[8tln]], [[3p7p]], [[3p7q]], [[3p7r]], [[3p7s]], [[3p7t]], [[3p7u]], [[3p7v]], [[3p7w]], [[3zi6]] – TML<br />
+
**[[2whz]], [[3fvp]], [[3dnz]], [[3do0]], [[3do1]], [[3do2]], [[2g4z]], [[2a7g]], [[1gxw]], [[1kei]], [[1l3f]], [[1tlx]], [[2tlx]], [[8tln]], [[3p7p]], [[3p7q]], [[3p7r]], [[3p7s]], [[3p7t]], [[3p7u]], [[3p7v]], [[3p7w]], [[3zi6]] – TML<br />
-
[[3ls7]], [[4ow3]] - TML residues 233-548<br />
+
**[[3ls7]], [[4ow3]] - TML residues 233-548<br />
-
[[1trl]] - TML residues 255-316 – NMR<br />
+
**[[1trl]] - TML residues 255-316 – NMR<br />
-
[[3fxs]], [[3fbo]], [[3eim]], [[1lna]], [[1lnb]], [[1lnc]], [[1lnd]], [[1lne]], [[1lnf]] - TML residues 233-548+metal ion replacing Ca<br />
+
**[[3fxs]], [[3fbo]], [[3eim]], [[1lna]], [[1lnb]], [[1lnc]], [[1lnd]], [[1lne]], [[1lnf]] - TML residues 233-548+metal ion replacing Ca<br />
-
=== TML+transition state analog ===
+
*Thermolysin TML+transition state analog
-
[[1tlp]], [[1tmn]], [[2tmn]], [[4tmn]], [[5tmn]] – TML+transition state analog<br />
+
**[[1tlp]], [[1tmn]], [[2tmn]], [[4tmn]], [[5tmn]] – TML+transition state analog<br />
-
===TML+ amino acid===
+
*Thermolysin + amino acid
-
[[1kl6]] – TML+ alanine<br />
+
**[[1kl6]] – TML+ alanine<br />
-
[[4m65]] – TML + asparagine<br />
+
**[[4m65]] – TML + asparagine<br />
-
[[3qgo]] – TML + phenylalanine methyl ester<br />
+
**[[3qgo]] – TML + phenylalanine methyl ester<br />
-
[[3qh1]] – TML + benzyloxycarboxyl- aspartate<br />
+
**[[3qh1]] – TML + benzyloxycarboxyl- aspartate<br />
-
[[3qh5]] – TML + benzyloxycarboxyl- aspartate + phenylalanine methyl ester
+
**[[3qh5]] – TML + benzyloxycarboxyl- aspartate + phenylalanine methyl ester
-
=== TML+ dipeptide ===
+
*Thermolysin + dipeptide
-
[[2wi0]], [[3tmn]] – TML+ dipeptide <br />
+
**[[2wi0]], [[3tmn]] – TML+ dipeptide <br />
-
[[3fgd]] - TML residues 233-548+dipeptide<br />
+
**[[3fgd]] - TML residues 233-548+dipeptide<br />
-
 
+
-
=== TML+inhibitor ===
+
-
 
+
-
[[1fjo]], [[1fj3]], [[1fjq]], [[1fjt]], [[1fju]], [[1fjv]], [[1fjw]], [[4tli]], [[5tli]], [[6tli]], [[7tli]], [[8tli]], [[1tli]], [[2tli]], [[3tli]] – TML soaked in organic solvents<br />
+
-
[[3fv4]], [[3fxp]], [[3flf]], [[3fb0]], [[3fcq]], [[3f28]], [[3f2p]], [[4mtw]], [[4mwp]], [[4mxj]], [[4mzn]], [[4n4e]], [[4n5p]], [[4n66]], [[4oi5]] – TML residues 233-548+inhibitor<br />
+
-
[[3fwd]], [[3for]], [[1y3g]], [[1zdp]], [[1z9g]], [[1pes]], [[1pe7]], [[1pe8]], [[1os0]], [[1kto]], [[1ks7]], [[1kro]], [[1kr6]], [[1kkk]], [[1kjo]], [[1kjp]], [[1qf0]], [[1qf1]], [[1qf2]], [[1hyt]], [[1thl]], [[6tmn]], [[7tln]], [[4tln]], [[5tln]], [[1pe5]], [[3ms3]], [[3msa]], [[3msf]], [[3msn]], [[3n21]], [[3nn7]], [[3t73]], [[3t74]], [[3t87]], [[3t8c]], [[3t8d]], [[3t8f]], [[3t8g]], [[3t8h]], [[4h57]], [[4d9w]], [[4d91]] – TML+inhibitor<br />
+
-
[[3ssb]] – TML + Impi-α<br />
+
-
[[3t2h]] – TML + trimethylamine oxide<br />
+
-
[[3t2i]] – TML + sarcosine<br />
+
-
[[3t2j]] – TML + betaine
+
-
 
+
-
</StructureSection>
+
-
==References==
+
*Thermolysin + inhibitor
-
<references />
+
-
[[Category:Topic Page]]
+
**[[1fjo]], [[1fj3]], [[1fjq]], [[1fjt]], [[1fju]], [[1fjv]], [[1fjw]], [[4tli]], [[5tli]], [[6tli]], [[7tli]], [[8tli]], [[1tli]], [[2tli]], [[3tli]] – TML soaked in organic solvents<br />
-
[[Category: Bacillus thermoproteolyticus]]
+
**[[3fv4]], [[3fxp]], [[3flf]], [[3fb0]], [[3fcq]], [[3f28]], [[3f2p]], [[4mtw]], [[4mwp]], [[4mxj]], [[4mzn]], [[4n4e]], [[4n5p]], [[4n66]], [[4oi5]] – TML residues 233-548+inhibitor<br />
-
[[Category: Single protein]]
+
**[[3fwd]], [[3for]], [[1y3g]], [[1zdp]], [[1z9g]], [[1pes]], [[1pe7]], [[1pe8]], [[1os0]], [[1kto]], [[1ks7]], [[1kro]], [[1kr6]], [[1kkk]], [[1kjo]], [[1kjp]], [[1qf0]], [[1qf1]], [[1qf2]], [[1hyt]], [[1thl]], [[6tmn]], [[7tln]], [[4tln]], [[5tln]], [[1pe5]], [[3ms3]], [[3msa]], [[3msf]], [[3msn]], [[3n21]], [[3nn7]], [[3t73]], [[3t74]], [[3t87]], [[3t8c]], [[3t8d]], [[3t8f]], [[3t8g]], [[3t8h]], [[4h57]], [[4d9w]], [[4d91]] – TML+inhibitor<br />
-
[[Category: Transaldolase]]
+
**[[3ssb]] – TML + Impi-α<br />
-
[[Category: Mueller-Dieckmann, C.]]
+
**[[3t2h]] – TML + trimethylamine oxide<br />
-
[[Category: Panjikar, S.]]
+
**[[3t2i]] – TML + sarcosine<br />
-
[[Category: Tucker, P A.]]
+
**[[3t2j]] – TML + betaine<br />
-
[[Category: Weiss, M S.]]
+
}}
-
[[Category: Transferase]]
+
[[Category: Topic Page]]
-
<br />
+
-
Created with the participation of [[User:Ralf Stephan|Ralf Stephan]].
+

Revision as of 10:51, 11 December 2014

Thermolysin complex with acetate, DMS, Zn+2 (grey) and Ca+2 (green) ions, 2a7g

Drag the structure with the mouse to rotate

3D Structures of Thermolysin

Updated on 11-December-2014

Proteopedia Page Contributors and Editors (what is this?)

Michal Harel, Alexander Berchansky

Retrieved from "http://52.214.119.220/wiki/index.php/Thermolysin"
Personal tools