Cas9
From Proteopedia
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* [[4un3]], [[4un4]], and [[4un5]] - ''S. pyogenes'' Cas9 bound to sgRNA and target DNA | * [[4un3]], [[4un4]], and [[4un5]] - ''S. pyogenes'' Cas9 bound to sgRNA and target DNA | ||
| - | [[Image:4un3 labeled.png|right]] | + | [[Image:4un3 labeled.png|right|390px]] |
* [[4oo8]] - ''S. pyogenes'' Cas9 bound to sgRNA and target DNA | * [[4oo8]] - ''S. pyogenes'' Cas9 bound to sgRNA and target DNA | ||
* [[4cmp]] | * [[4cmp]] | ||
Revision as of 05:25, 13 December 2014
Cas9 is the RNA-guided DNA endonuclease used by the CRISPR(clustered regularly interspaced short palindromic repeats)-associated systems to generate double-strand DNA breaks in the invading DNA during an adaptive bacterial immune response.
The CRISPR-associated endonuclease Cas9 has been exploited for use in genome editing systems. In such systems, an engineered single-guide RNA (sgRNA) is used to target double-stranded breaks in genomic DNA. Depending on what repair pathway is triggered, often dictated by the inclusion of additional engineered components, the targeted site either is disrupted or incorporates additional genetic sequences.
Articles in Proteopedia concerning Cas9 include:
- any?
Contents |
3D structures of Cas9
Streptococcus pyogenes Cas9
Actinomyces naeslundii Cas9
See Also
References
Proteopedia Page Contributors and Editors (what is this?)
Wayne Decatur, Michal Harel, Karsten Theis, Joel L. Sussman, Ann Taylor, Thomas Gastineau
