Ubiquitin activating enzyme

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'''Ubiquitin activating enzyme''' (Uba) or '''E1 enzyme''' catalyzes the first step in the ubiquitination of a protein tagged to be degraded by the proteasome. Ubiquitin activating enzyme (E1) and ATP produce a C-terminal acyl adenylated ubiquitin molecule which binds to ubiquitin conjugating enzyme (Ubc) cysteine. The Ubc then binds to ubiquitin ligase (E3) via a conserved binding region. E3 catalyzes the transfer of ubiquitin from the Ubc-ubiquitin complex to a lysine residue of the target protein. '''Uba3''' is the catalytic subunit of NEDD8 activating enzyme and APPB1 is its regulatory subunit. Similar to ubiquitin and SUMO, NEDD8 binds to proteins after processing of its C-terminal.
'''Ubiquitin activating enzyme''' (Uba) or '''E1 enzyme''' catalyzes the first step in the ubiquitination of a protein tagged to be degraded by the proteasome. Ubiquitin activating enzyme (E1) and ATP produce a C-terminal acyl adenylated ubiquitin molecule which binds to ubiquitin conjugating enzyme (Ubc) cysteine. The Ubc then binds to ubiquitin ligase (E3) via a conserved binding region. E3 catalyzes the transfer of ubiquitin from the Ubc-ubiquitin complex to a lysine residue of the target protein. '''Uba3''' is the catalytic subunit of NEDD8 activating enzyme and APPB1 is its regulatory subunit. Similar to ubiquitin and SUMO, NEDD8 binds to proteins after processing of its C-terminal.
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</StructureSection>
== 3D Structures of ubiquitin activating enzyme ==
== 3D Structures of ubiquitin activating enzyme ==
Updated on {{REVISIONDAY2}}-{{MONTHNAME|{{REVISIONMONTH}}}}-{{REVISIONYEAR}}
Updated on {{REVISIONDAY2}}-{{MONTHNAME|{{REVISIONMONTH}}}}-{{REVISIONYEAR}}
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{{#tree:id=OrganizedByTopic|openlevels=0|
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[[1z7l]] – mUba 1 second catalytic Cys half-domain - mouse <BR />
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*Ubiquitin activating enzyme 1
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[[2v31]] – mUba X first catalytic Cys half-domain <BR />
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===Uba1===
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**[[1z7l]] – mUba 1 second catalytic Cys half-domain - mouse <BR />
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**[[2v31]] – mUba X first catalytic Cys half-domain <BR />
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**[[3cmm]], [[4nnj]] – yUba + ubiquitin – yeast <BR />
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**[[4ii2]] – hUba + E2 Ubc4 + ubiquitin-ribosomal protein L40 + ATP <BR />
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**[[4ii3]] – hUba + ubiquitin-ribosomal protein L40 + ATP <BR />
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[[3cmm]], [[4nnj]] – yUba + ubiquitin – yeast <BR />
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*Ubiquitin activating enzyme 2
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[[4ii2]] – hUba + E2 Ubc4 + ubiquitin-ribosomal protein L40 + ATP <BR />
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[[4ii3]] – hUba + ubiquitin-ribosomal protein L40 + ATP <BR />
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===Uba2===
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**[[3ong]], [[3onh]] – yUba + SUMO-conjugating enzyme Ubc9 <BR />
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[[3ong]], [[3onh]] – yUba + SUMO-conjugating enzyme Ubc9 <BR />
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*NEDD8-activating enzyme ubiquitin activating enzyme 3 subunit
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===NEDD8-activating enzyme Uba3 subunit===
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**[[2lq7]] – hUba – human <BR />
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**[[1r4m]] – hUba + NEDD8 <BR />
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**[[1r4n]] – hUba (mutant) + NEDD8 + APPBP1 + ATP <BR />
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**[[1tt5]] – hUba + E2 Ubc12 + APPBP1 <BR />
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**[[1y8x]] – hUba + E2 Ubc12 <BR />
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**[[1yov]] – hUba + APPBP1 <BR />
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**[[3dbr]] – hUba + APPBP1 (mutant) + NEDD8 <BR />
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**[[2nvu]] – hUba + E2 Ubc12 + APPBP1 + NEDD8 + ATP <BR />
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**[[3gzn]] – hUba + inhibitor + APPBP1 + NEDD8 + ATP <BR />
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**[[3dbh]], [[3dbl]] – hUba (mutant) + NEDD8 <BR />
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**[[3fn1]] – hUba (mutant) + NEDD8-conjugating enzyme <BR />
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[[2lq7]] – hUba – human <BR />
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*Ubiquitin activating enzyme 5
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[[1r4m]] – hUba + NEDD8 <BR />
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[[1r4n]] – hUba (mutant) + NEDD8 + APPBP1 + ATP <BR />
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[[1tt5]] – hUba + E2 Ubc12 + APPBP1 <BR />
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[[1y8x]] – hUba + E2 Ubc12 <BR />
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[[1yov]] – hUba + APPBP1 <BR />
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[[3dbr]] – hUba + APPBP1 (mutant) + NEDD8 <BR />
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[[2nvu]] – hUba + E2 Ubc12 + APPBP1 + NEDD8 + ATP <BR />
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[[3gzn]] – hUba + inhibitor + APPBP1 + NEDD8 + ATP <BR />
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[[3dbh]], [[3dbl]] – hUba (mutant) + NEDD8 <BR />
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[[3fn1]] – hUba (mutant) + NEDD8-conjugating enzyme <BR />
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===Uba5===
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**[[3guc]] – hUba + AMPPNP <BR />
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**[[3h8v]] – hUba + ATP <BR />
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[[3guc]] – hUba + AMPPNP <BR />
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*SUMO-activating enzyme
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[[3h8v]] – hUba + ATP <BR />
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===SUMO-activating enzyme===
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**[[3kyc]] – hSua subunits 1,2 + SUMO <BR />
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**[[3kyd]] – hSua subunits 1,2 + SUMO + AMP <BR />
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[[3kyc]] – hSua subunits 1,2 + SUMO <BR />
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}}
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[[3kyd]] – hSua subunits 1,2 + SUMO + AMP <BR />
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[[Category:Topic Page]]
[[Category:Topic Page]]

Revision as of 09:37, 16 December 2014

Structure of human ubiquitin activating enzyme Uba3 subunit (green) complex with E2 Ubc12 (grey) (PDB entry 1y8x)

Drag the structure with the mouse to rotate

3D Structures of ubiquitin activating enzyme

Updated on 16-December-2014

Proteopedia Page Contributors and Editors (what is this?)

Michal Harel, Alexander Berchansky

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