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4ok9

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{{STRUCTURE_4ok9| PDB=4ok9 | SCENE= }}
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==Crystal structure of the single-stranded RNA binding protein HutP from Geobacillus thermodenitrificans==
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===Crystal structure of the single-stranded RNA binding protein HutP from Geobacillus thermodenitrificans===
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<StructureSection load='4ok9' size='340' side='right' caption='[[4ok9]], [[Resolution|resolution]] 1.91&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[4ok9]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Geotn Geotn]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4OK9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4OK9 FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HIS:HISTIDINE'>HIS</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4okq|4okq]]</td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">GTNG_0361, hutP ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=420246 GEOTN])</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ok9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ok9 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4ok9 RCSB], [http://www.ebi.ac.uk/pdbsum/4ok9 PDBsum]</span></td></tr>
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</table>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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RNA binding proteins control gene expression by the attenuation/antitermination mechanism. HutP is an RNA binding antitermination protein. It regulates the expression of hut operon when it binds with RNA by modulating the secondary structure of single-stranded hut mRNA. HutP necessitates the presence of l-histidine and divalent metal ion to bind with RNA. Herein, we report the crystal structures of ternary complex (HutP-l-histidine-Mg(2+)) and EDTA (0.5 M) treated ternary complex (HutP-l-histidine-Mg(2+)), solved at 1.9 A and 2.5 A resolutions, respectively, from Geobacillus thermodenitrificans. The addition of 0.5 M EDTA does not affect the overall metal-ion mediated ternary complex structure and however, the metal ions at the non-specific binding sites are chelated, as evidenced from the results of structural features.
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==Function==
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Crystal structure of the single-stranded RNA binding protein HutP from Geobacillus thermodenitrificans.,Thiruselvam V, Sivaraman P, Kumarevel T, Ponnuswamy MN Biochem Biophys Res Commun. 2014 Apr 18;446(4):945-51. doi:, 10.1016/j.bbrc.2014.03.036. Epub 2014 Mar 17. PMID:24650662<ref>PMID:24650662</ref>
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[[http://www.uniprot.org/uniprot/HUTP_GEOTN HUTP_GEOTN]] Antiterminator that binds to cis-acting regulatory sequences on the mRNA in the presence of histidine, thereby suppressing transcription termination and activating the hut operon for histidine utilization (By similarity).
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==About this Structure==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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[[4ok9]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4OK9 OCA].
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</div>
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[[Category: Kumarevel, T S.]]
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== References ==
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[[Category: Ponnuswamy, M N.]]
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<references/>
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[[Category: Thiruselvam, V.]]
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__TOC__
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</StructureSection>
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[[Category: Geotn]]
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[[Category: Kumarevel, T S]]
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[[Category: Ponnuswamy, M N]]
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[[Category: Thiruselvam, V]]
[[Category: Antitermination]]
[[Category: Antitermination]]
[[Category: Rna binding protein]]
[[Category: Rna binding protein]]
[[Category: Single stranded rna binding protein]]
[[Category: Single stranded rna binding protein]]

Revision as of 09:31, 17 December 2014

Crystal structure of the single-stranded RNA binding protein HutP from Geobacillus thermodenitrificans

4ok9, resolution 1.91Å

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