4ok9
From Proteopedia
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| - | + | ==Crystal structure of the single-stranded RNA binding protein HutP from Geobacillus thermodenitrificans== | |
| - | + | <StructureSection load='4ok9' size='340' side='right' caption='[[4ok9]], [[Resolution|resolution]] 1.91Å' scene=''> | |
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[4ok9]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Geotn Geotn]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4OK9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4OK9 FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HIS:HISTIDINE'>HIS</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> | ||
| + | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4okq|4okq]]</td></tr> | ||
| + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">GTNG_0361, hutP ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=420246 GEOTN])</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ok9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ok9 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4ok9 RCSB], [http://www.ebi.ac.uk/pdbsum/4ok9 PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | RNA binding proteins control gene expression by the attenuation/antitermination mechanism. HutP is an RNA binding antitermination protein. It regulates the expression of hut operon when it binds with RNA by modulating the secondary structure of single-stranded hut mRNA. HutP necessitates the presence of l-histidine and divalent metal ion to bind with RNA. Herein, we report the crystal structures of ternary complex (HutP-l-histidine-Mg(2+)) and EDTA (0.5 M) treated ternary complex (HutP-l-histidine-Mg(2+)), solved at 1.9 A and 2.5 A resolutions, respectively, from Geobacillus thermodenitrificans. The addition of 0.5 M EDTA does not affect the overall metal-ion mediated ternary complex structure and however, the metal ions at the non-specific binding sites are chelated, as evidenced from the results of structural features. | ||
| - | + | Crystal structure of the single-stranded RNA binding protein HutP from Geobacillus thermodenitrificans.,Thiruselvam V, Sivaraman P, Kumarevel T, Ponnuswamy MN Biochem Biophys Res Commun. 2014 Apr 18;446(4):945-51. doi:, 10.1016/j.bbrc.2014.03.036. Epub 2014 Mar 17. PMID:24650662<ref>PMID:24650662</ref> | |
| - | + | ||
| - | == | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> |
| - | [[ | + | </div> |
| - | [[Category: Kumarevel, T S | + | == References == |
| - | [[Category: Ponnuswamy, M N | + | <references/> |
| - | [[Category: Thiruselvam, V | + | __TOC__ |
| + | </StructureSection> | ||
| + | [[Category: Geotn]] | ||
| + | [[Category: Kumarevel, T S]] | ||
| + | [[Category: Ponnuswamy, M N]] | ||
| + | [[Category: Thiruselvam, V]] | ||
[[Category: Antitermination]] | [[Category: Antitermination]] | ||
[[Category: Rna binding protein]] | [[Category: Rna binding protein]] | ||
[[Category: Single stranded rna binding protein]] | [[Category: Single stranded rna binding protein]] | ||
Revision as of 09:31, 17 December 2014
Crystal structure of the single-stranded RNA binding protein HutP from Geobacillus thermodenitrificans
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