1ox7
From Proteopedia
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| - | [[Image:1ox7.gif|left|200px]] | + | [[Image:1ox7.gif|left|200px]] |
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| - | '''Crystal structure of yeast cytosine deaminase apo-enzyme: inorganic zinc bound''' | + | {{Structure |
| + | |PDB= 1ox7 |SIZE=350|CAPTION= <scene name='initialview01'>1ox7</scene>, resolution 1.43Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene> and <scene name='pdbligand=CA:CALCIUM ION'>CA</scene> | ||
| + | |ACTIVITY= [http://en.wikipedia.org/wiki/Cytosine_deaminase Cytosine deaminase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.4.1 3.5.4.1] | ||
| + | |GENE= FCY1 OR YPR062W OR YP9499.17 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 Saccharomyces cerevisiae]) | ||
| + | }} | ||
| + | |||
| + | '''Crystal structure of yeast cytosine deaminase apo-enzyme: inorganic zinc bound''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1OX7 is a [ | + | 1OX7 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OX7 OCA]. |
==Reference== | ==Reference== | ||
| - | The 1.14 A crystal structure of yeast cytosine deaminase: evolution of nucleotide salvage enzymes and implications for genetic chemotherapy., Ireton GC, Black ME, Stoddard BL, Structure. 2003 Aug;11(8):961-72. PMID:[http:// | + | The 1.14 A crystal structure of yeast cytosine deaminase: evolution of nucleotide salvage enzymes and implications for genetic chemotherapy., Ireton GC, Black ME, Stoddard BL, Structure. 2003 Aug;11(8):961-72. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12906827 12906827] |
[[Category: Cytosine deaminase]] | [[Category: Cytosine deaminase]] | ||
[[Category: Saccharomyces cerevisiae]] | [[Category: Saccharomyces cerevisiae]] | ||
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[[Category: aminohydrolase]] | [[Category: aminohydrolase]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 13:16:49 2008'' |
Revision as of 11:16, 20 March 2008
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| , resolution 1.43Å | |||||||
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| Ligands: | and | ||||||
| Gene: | FCY1 OR YPR062W OR YP9499.17 (Saccharomyces cerevisiae) | ||||||
| Activity: | Cytosine deaminase, with EC number 3.5.4.1 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal structure of yeast cytosine deaminase apo-enzyme: inorganic zinc bound
Overview
Cytosine deaminase (CD) catalyzes the deamination of cytosine and is only present in prokaryotes and fungi, where it is a member of the pyrimidine salvage pathway. The enzyme is of interest both for antimicrobial drug design and gene therapy applications against tumors. The structure of Saccharomyces cerevisiae CD has been determined in the presence and absence of a mechanism-based inhibitor, at 1.14 and 1.43 A resolution, respectively. The enzyme forms an alpha/beta fold similar to bacterial cytidine deaminase, but with no similarity to the alpha/beta barrel fold used by bacterial cytosine deaminase or mammalian adenosine deaminase. The structures observed for bacterial, fungal, and mammalian nucleic acid deaminases represent an example of the parallel evolution of two unique protein folds to carry out the same reaction on a diverse array of substrates.
About this Structure
1OX7 is a Single protein structure of sequence from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.
Reference
The 1.14 A crystal structure of yeast cytosine deaminase: evolution of nucleotide salvage enzymes and implications for genetic chemotherapy., Ireton GC, Black ME, Stoddard BL, Structure. 2003 Aug;11(8):961-72. PMID:12906827
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