1ox9
From Proteopedia
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| - | [[Image:1ox9.gif|left|200px]] | + | [[Image:1ox9.gif|left|200px]] |
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| - | '''Crystal structure of SspB-ssrA complex''' | + | {{Structure |
| + | |PDB= 1ox9 |SIZE=350|CAPTION= <scene name='initialview01'>1ox9</scene>, resolution 2.9Å | ||
| + | |SITE= | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= | ||
| + | |GENE= SSPB OR B3228 OR Z4586 OR ECS4101 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=168807 Escherichia coli O127:H6]) | ||
| + | }} | ||
| + | |||
| + | '''Crystal structure of SspB-ssrA complex''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1OX9 is a [ | + | 1OX9 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli_o127:h6 Escherichia coli o127:h6]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OX9 OCA]. |
==Reference== | ==Reference== | ||
| - | Structural basis of degradation signal recognition by SspB, a specificity-enhancing factor for the ClpXP proteolytic machine., Song HK, Eck MJ, Mol Cell. 2003 Jul;12(1):75-86. PMID:[http:// | + | Structural basis of degradation signal recognition by SspB, a specificity-enhancing factor for the ClpXP proteolytic machine., Song HK, Eck MJ, Mol Cell. 2003 Jul;12(1):75-86. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12887894 12887894] |
[[Category: Escherichia coli o127:h6]] | [[Category: Escherichia coli o127:h6]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: sspb-ssra]] | [[Category: sspb-ssra]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 13:16:51 2008'' |
Revision as of 11:16, 20 March 2008
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| , resolution 2.9Å | |||||||
|---|---|---|---|---|---|---|---|
| Gene: | SSPB OR B3228 OR Z4586 OR ECS4101 (Escherichia coli O127:H6) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal structure of SspB-ssrA complex
Overview
In prokaryotes, incomplete or misfolded polypeptides emanating from a stalled ribosome are marked for degradation by the addition of an 11 residue peptide (AANDENYALAA) to their C terminus. Substrates containing this conserved degradation signal, the SsrA tag, are targeted to specific proteases including ClpXP and ClpAP. SspB was originally characterized as a stringent starvation protein and has been found to bind specifically to SsrA-tagged proteins and to enhance recognition of these proteins by the ClpXP degradation machine. Here, we report the crystal structures of SspB alone and in complex with an SsrA peptide. Unexpectedly, SspB exhibits a fold found in Sm-family RNA binding proteins. The dimeric SspB structures explain the key determinants for recognition of the SsrA tag and define a hydrophobic channel that may bind unfolded substrates.
About this Structure
1OX9 is a Single protein structure of sequence from Escherichia coli o127:h6. Full crystallographic information is available from OCA.
Reference
Structural basis of degradation signal recognition by SspB, a specificity-enhancing factor for the ClpXP proteolytic machine., Song HK, Eck MJ, Mol Cell. 2003 Jul;12(1):75-86. PMID:12887894
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