1oxc
From Proteopedia
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| - | [[Image:1oxc.jpg|left|200px]] | + | [[Image:1oxc.jpg|left|200px]] |
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| - | '''LecB (PA-LII) in complex with FUCOSE''' | + | {{Structure |
| + | |PDB= 1oxc |SIZE=350|CAPTION= <scene name='initialview01'>1oxc</scene>, resolution 1.2Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=FUL:BETA-L-FUCOSE'>FUL</scene>, <scene name='pdbligand=FUC:ALPHA-L-FUCOSE'>FUC</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene> and <scene name='pdbligand=SO4:SULFATE ION'>SO4</scene> | ||
| + | |ACTIVITY= | ||
| + | |GENE= LecB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=287 Pseudomonas aeruginosa]) | ||
| + | }} | ||
| + | |||
| + | '''LecB (PA-LII) in complex with FUCOSE''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1OXC is a [ | + | 1OXC is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OXC OCA]. |
==Reference== | ==Reference== | ||
| - | Structural basis of carbohydrate recognition by the lectin LecB from Pseudomonas aeruginosa., Loris R, Tielker D, Jaeger KE, Wyns L, J Mol Biol. 2003 Aug 22;331(4):861-70. PMID:[http:// | + | Structural basis of carbohydrate recognition by the lectin LecB from Pseudomonas aeruginosa., Loris R, Tielker D, Jaeger KE, Wyns L, J Mol Biol. 2003 Aug 22;331(4):861-70. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12909014 12909014] |
[[Category: Pseudomonas aeruginosa]] | [[Category: Pseudomonas aeruginosa]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: lectin]] | [[Category: lectin]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 13:16:53 2008'' |
Revision as of 11:16, 20 March 2008
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| , resolution 1.2Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , , and | ||||||
| Gene: | LecB (Pseudomonas aeruginosa) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
LecB (PA-LII) in complex with FUCOSE
Overview
The crystal structure of Pseudomonas aeruginosa fucose-specific lectin LecB was determined in its metal-bound and metal-free state as well as in complex with fucose, mannose and fructopyranose. All three monosaccharides bind isosterically via direct interactions with two calcium ions as well as direct hydrogen bonds with several side-chains. The higher affinity for fucose is explained by the details of the binding site around C6 and O1 of fucose. In the mannose and fructose complexes, a carboxylate oxygen atom and one or two hydroxyl groups are partly shielded from solvent upon sugar binding, preventing them from completely fulfilling their hydrogen bonding potential. In the fucose complex, no such defects are observed. Instead, C6 makes favourable interactions with a small hydrophobic patch. Upon demetallization, the C terminus as well as the otherwise rigid metal-binding loop become more mobile and adopt multiple conformations.
About this Structure
1OXC is a Single protein structure of sequence from Pseudomonas aeruginosa. Full crystallographic information is available from OCA.
Reference
Structural basis of carbohydrate recognition by the lectin LecB from Pseudomonas aeruginosa., Loris R, Tielker D, Jaeger KE, Wyns L, J Mol Biol. 2003 Aug 22;331(4):861-70. PMID:12909014
Page seeded by OCA on Thu Mar 20 13:16:53 2008
Categories: Pseudomonas aeruginosa | Single protein | Jaeger, K E. | Loris, R. | Tielker, D. | Wyns, L. | CA | FUC | FUL | SO4 | Carbohydrate | Lectin
