1oyj
From Proteopedia
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| - | [[Image:1oyj.jpg|left|200px]] | + | [[Image:1oyj.jpg|left|200px]] |
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| - | '''Crystal structure solution of Rice GST1 (OsGSTU1) in complex with glutathione.''' | + | {{Structure |
| + | |PDB= 1oyj |SIZE=350|CAPTION= <scene name='initialview01'>1oyj</scene>, resolution 1.95Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GTT:GLUTATHIONE'>GTT</scene>, <scene name='pdbligand=GSW:D-GAMMA-GLUTAMYL-L-CYSTEINYLGLYCINE'>GSW</scene> and <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene> | ||
| + | |ACTIVITY= [http://en.wikipedia.org/wiki/Glutathione_transferase Glutathione transferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.18 2.5.1.18] | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''Crystal structure solution of Rice GST1 (OsGSTU1) in complex with glutathione.''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1OYJ is a [ | + | 1OYJ is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Oryza_sativa Oryza sativa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OYJ OCA]. |
==Reference== | ==Reference== | ||
| - | Forced evolution of a herbicide detoxifying glutathione transferase., Dixon DP, McEwen AG, Lapthorn AJ, Edwards R, J Biol Chem. 2003 Jun 27;278(26):23930-5. Epub 2003 Apr 11. PMID:[http:// | + | Forced evolution of a herbicide detoxifying glutathione transferase., Dixon DP, McEwen AG, Lapthorn AJ, Edwards R, J Biol Chem. 2003 Jun 27;278(26):23930-5. Epub 2003 Apr 11. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12692133 12692133] |
[[Category: Glutathione transferase]] | [[Category: Glutathione transferase]] | ||
[[Category: Oryza sativa]] | [[Category: Oryza sativa]] | ||
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[[Category: plant glutathione s-transferase]] | [[Category: plant glutathione s-transferase]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 13:17:28 2008'' |
Revision as of 11:17, 20 March 2008
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| , resolution 1.95Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , , , and | ||||||
| Activity: | Glutathione transferase, with EC number 2.5.1.18 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal structure solution of Rice GST1 (OsGSTU1) in complex with glutathione.
Overview
Plant Tau class glutathione transferases (GSTUs) detoxify diphenylether herbicides such as fluorodifen, determining their selectivity in crops and weeds. Using reconstructive PCR, a series of mutant GSTUs were generated from in vitro recombination and mutagenesis of the maize sequences ZmGSTU1 and ZmGSTU2 (with the prefix Zm designating Zea mays L.). A screen of 5000 mutant GSTUs identified seven enzymes with enhanced fluorodifen detoxifying activity. The best performing enhanced fluorodifen detoxifying mutant (EFD) had activity 19-fold higher than the parent enzymes, with a single point mutation conferring this enhancement. Further mutagenesis of this residue generated an EFD with a 29-fold higher catalytic efficiency toward fluorodifen as compared with the parents but with unaltered catalysis toward other substrates. When expressed in Arabidopsis thaliana, the optimized EFD, but not the parent enzymes, conferred enhanced tolerance to fluorodifen. Molecular modeling predicts that the serendipitous mutation giving the improvement in detoxification is due to the removal of an unfavorable interaction together with the introduction of a favorable change in conformation of residues 107-119, which contribute to herbicide binding.
About this Structure
1OYJ is a Single protein structure of sequence from Oryza sativa. Full crystallographic information is available from OCA.
Reference
Forced evolution of a herbicide detoxifying glutathione transferase., Dixon DP, McEwen AG, Lapthorn AJ, Edwards R, J Biol Chem. 2003 Jun 27;278(26):23930-5. Epub 2003 Apr 11. PMID:12692133
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