1oyy
From Proteopedia
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| - | [[Image:1oyy.jpg|left|200px]] | + | [[Image:1oyy.jpg|left|200px]] |
| - | + | ||
| - | '''Structure of the RecQ Catalytic Core bound to ATP-gamma-S''' | + | {{Structure |
| + | |PDB= 1oyy |SIZE=350|CAPTION= <scene name='initialview01'>1oyy</scene>, resolution 2.5Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene> and <scene name='pdbligand=AGS:PHOSPHOTHIOPHOSPHORIC ACID-ADENYLATE ESTER'>AGS</scene> | ||
| + | |ACTIVITY= | ||
| + | |GENE= recQ ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli]) | ||
| + | }} | ||
| + | |||
| + | '''Structure of the RecQ Catalytic Core bound to ATP-gamma-S''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1OYY is a [ | + | 1OYY is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OYY OCA]. |
==Reference== | ==Reference== | ||
| - | High-resolution structure of the E.coli RecQ helicase catalytic core., Bernstein DA, Zittel MC, Keck JL, EMBO J. 2003 Oct 1;22(19):4910-21. PMID:[http:// | + | High-resolution structure of the E.coli RecQ helicase catalytic core., Bernstein DA, Zittel MC, Keck JL, EMBO J. 2003 Oct 1;22(19):4910-21. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/14517231 14517231] |
[[Category: Escherichia coli]] | [[Category: Escherichia coli]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: ZN]] | [[Category: ZN]] | ||
[[Category: atp binding]] | [[Category: atp binding]] | ||
| - | [[Category: atp(gamma) | + | [[Category: atp(gamma)]] |
[[Category: helicase]] | [[Category: helicase]] | ||
[[Category: helix-turn-helix]] | [[Category: helix-turn-helix]] | ||
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[[Category: zn(2+) binding]] | [[Category: zn(2+) binding]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 13:17:32 2008'' |
Revision as of 11:17, 20 March 2008
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| , resolution 2.5Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , and | ||||||
| Gene: | recQ (Escherichia coli) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Structure of the RecQ Catalytic Core bound to ATP-gamma-S
Overview
RecQ family helicases catalyze critical genome maintenance reactions in bacterial and eukaryotic cells, playing key roles in several DNA metabolic processes. Mutations in recQ genes are linked to genome instability and human disease. To define the physical basis of RecQ enzyme function, we have determined a 1.8 A resolution crystal structure of the catalytic core of Escherichia coli RecQ in its unbound form and a 2.5 A resolution structure of the core bound to the ATP analog ATPgammaS. The RecQ core comprises four conserved subdomains; two of these combine to form its helicase region, while the others form unexpected Zn(2+)-binding and winged-helix motifs. The structures reveal the molecular basis of missense mutations that cause Bloom's syndrome, a human RecQ-associated disease. Finally, based on findings from the structures, we propose a mechanism for RecQ activity that could explain its functional coordination with topoisomerase III.
About this Structure
1OYY is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.
Reference
High-resolution structure of the E.coli RecQ helicase catalytic core., Bernstein DA, Zittel MC, Keck JL, EMBO J. 2003 Oct 1;22(19):4910-21. PMID:14517231
Page seeded by OCA on Thu Mar 20 13:17:32 2008
Categories: Escherichia coli | Single protein | Bernstein, D A. | Keck, J L. | Zittel, M C. | AGS | MN | ZN | Atp binding | Atp(gamma) | Helicase | Helix-turn-helix | Recq | Winged helix | Zn(2+) binding
