1p16

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
[[Image:1p16.gif|left|200px]]<br /><applet load="1p16" size="350" color="white" frame="true" align="right" spinBox="true"
+
[[Image:1p16.gif|left|200px]]
-
caption="1p16, resolution 2.70&Aring;" />
+
 
-
'''Structure of an mRNA capping enzyme bound to the phosphorylated carboxyl-terminal domain of RNA polymerase II'''<br />
+
{{Structure
 +
|PDB= 1p16 |SIZE=350|CAPTION= <scene name='initialview01'>1p16</scene>, resolution 2.70&Aring;
 +
|SITE=
 +
|LIGAND= <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=G:GUANOSINE-5'-MONOPHOSPHATE'>G</scene> and <scene name='pdbligand=GTP:GUANOSINE-5'-TRIPHOSPHATE'>GTP</scene>
 +
|ACTIVITY= [http://en.wikipedia.org/wiki/mRNA_guanylyltransferase mRNA guanylyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.50 2.7.7.50]
 +
|GENE= CGT1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5476 Candida albicans])
 +
}}
 +
 
 +
'''Structure of an mRNA capping enzyme bound to the phosphorylated carboxyl-terminal domain of RNA polymerase II'''
 +
 
==Overview==
==Overview==
Line 7: Line 16:
==About this Structure==
==About this Structure==
-
1P16 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Candida_albicans Candida albicans] with <scene name='pdbligand=PO4:'>PO4</scene>, <scene name='pdbligand=G:'>G</scene> and <scene name='pdbligand=GTP:'>GTP</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/mRNA_guanylyltransferase mRNA guanylyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.50 2.7.7.50] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1P16 OCA].
+
1P16 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Candida_albicans Candida albicans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1P16 OCA].
==Reference==
==Reference==
-
Structure of an mRNA capping enzyme bound to the phosphorylated carboxy-terminal domain of RNA polymerase II., Fabrega C, Shen V, Shuman S, Lima CD, Mol Cell. 2003 Jun;11(6):1549-61. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12820968 12820968]
+
Structure of an mRNA capping enzyme bound to the phosphorylated carboxy-terminal domain of RNA polymerase II., Fabrega C, Shen V, Shuman S, Lima CD, Mol Cell. 2003 Jun;11(6):1549-61. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12820968 12820968]
[[Category: Candida albicans]]
[[Category: Candida albicans]]
[[Category: Single protein]]
[[Category: Single protein]]
Line 21: Line 30:
[[Category: GTP]]
[[Category: GTP]]
[[Category: PO4]]
[[Category: PO4]]
-
[[Category: candida albicans]]
+
[[Category: candida albican]]
[[Category: capping]]
[[Category: capping]]
[[Category: ctd]]
[[Category: ctd]]
Line 29: Line 38:
[[Category: transcription]]
[[Category: transcription]]
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:23:58 2008''
+
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 13:18:23 2008''

Revision as of 11:18, 20 March 2008

Image:1p16.gif


PDB ID 1p16

Drag the structure with the mouse to rotate
, resolution 2.70Å
Ligands: , and
Gene: CGT1 (Candida albicans)
Activity: mRNA guanylyltransferase, with EC number 2.7.7.50
Coordinates: save as pdb, mmCIF, xml



Structure of an mRNA capping enzyme bound to the phosphorylated carboxyl-terminal domain of RNA polymerase II


Overview

The 2.7 A structure of Candida albicans RNA guanylyltransferase Cgt1 cocrystallized with a carboxy-terminal domain (CTD) peptide composed of four Ser5-PO4 YSPTSPS heptad repeats illuminates distinct CTD-docking sites localized to the Cgt1 N-terminal nucleotidyl transferase domain. Tyr1, Pro3, Pro6, and Ser5-PO4 side chains from each of two YSPTSPS repeats contribute to the interface. Comparison to the Pin1-CTD structure shows that the CTD can assume markedly different conformations that are templated by particular binding partners. Structural plasticity combined with remodeling of CTD primary structure by kinases and phosphatases provides a versatile mechanism by which the CTD can recruit structurally dissimilar proteins during transcription. A binding site for the RNA triphosphatase component of the capping apparatus was also uncovered within the Cgt1 OB domain.

About this Structure

1P16 is a Single protein structure of sequence from Candida albicans. Full crystallographic information is available from OCA.

Reference

Structure of an mRNA capping enzyme bound to the phosphorylated carboxy-terminal domain of RNA polymerase II., Fabrega C, Shen V, Shuman S, Lima CD, Mol Cell. 2003 Jun;11(6):1549-61. PMID:12820968

Page seeded by OCA on Thu Mar 20 13:18:23 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1p16"
Personal tools