1y0q
From Proteopedia
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| - | + | ==Crystal structure of an active group I ribozyme-product complex== | |
| - | + | <StructureSection load='1y0q' size='340' side='right' caption='[[1y0q]], [[Resolution|resolution]] 3.60Å' scene=''> | |
| - | + | == Structural highlights == | |
| + | <table><tr><td colspan='2'>[[1y0q]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Staphylococcus_phage_twort Staphylococcus phage twort]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Y0Q OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1Y0Q FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=SPK:SPERMINE+(FULLY+PROTONATED+FORM)'>SPK</scene></td></tr> | ||
| + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">orf142-I2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=55510 Staphylococcus phage Twort])</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1y0q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1y0q OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1y0q RCSB], [http://www.ebi.ac.uk/pdbsum/1y0q PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Group I introns are catalytic RNAs capable of orchestrating two sequential phosphotransesterification reactions that result in self-splicing. To understand how the group I intron active site facilitates catalysis, we have solved the structure of an active ribozyme derived from the orf142-I2 intron from phage Twort bound to a four-nucleotide product RNA at a resolution of 3.6 A. In addition to the three conserved domains characteristic of all group I introns, the Twort ribozyme has peripheral insertions characteristic of phage introns. These elements form a ring that completely envelops the active site, where a snug pocket for guanosine is formed by a series of stacked base triples. The structure of the active site reveals three potential binding sites for catalytic metals, and invokes a role for the 2' hydroxyl of the guanosine substrate in organization of the active site for catalysis. | ||
| - | + | Crystal structure of a phage Twort group I ribozyme-product complex.,Golden BL, Kim H, Chase E Nat Struct Mol Biol. 2005 Jan;12(1):82-9. Epub 2004 Dec 5. PMID:15580277<ref>PMID:15580277</ref> | |
| - | + | ||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
==See Also== | ==See Also== | ||
*[[Group I intron|Group I intron]] | *[[Group I intron|Group I intron]] | ||
*[[Ribozyme|Ribozyme]] | *[[Ribozyme|Ribozyme]] | ||
| - | + | == References == | |
| - | == | + | <references/> |
| - | < | + | __TOC__ |
| + | </StructureSection> | ||
[[Category: Staphylococcus phage twort]] | [[Category: Staphylococcus phage twort]] | ||
| - | [[Category: Chase, E | + | [[Category: Chase, E]] |
| - | [[Category: Golden, B L | + | [[Category: Golden, B L]] |
| - | [[Category: Kim, H | + | [[Category: Kim, H]] |
[[Category: Guanosine-binding]] | [[Category: Guanosine-binding]] | ||
[[Category: Ribozyme]] | [[Category: Ribozyme]] | ||
Revision as of 13:43, 17 December 2014
Crystal structure of an active group I ribozyme-product complex
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