2ksy
From Proteopedia
(Difference between revisions)
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| - | + | ==Solution nmr structure of sensory rhodopsin II== | |
| - | + | <StructureSection load='2ksy' size='340' side='right' caption='[[2ksy]], [[NMR_Ensembles_of_Models | 30 NMR models]]' scene=''> | |
| - | === | + | == Structural highlights == |
| - | + | <table><tr><td colspan='2'>[[2ksy]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Natronomonas_pharaonis Natronomonas pharaonis]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KSY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2KSY FirstGlance]. <br> | |
| + | </td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=LYR:N~6~-[(2Z,4E,6E,8E)-3,7-DIMETHYL-9-(2,6,6-TRIMETHYLCYCLOHEX-1-EN-1-YL)NONA-2,4,6,8-TETRAENYL]LYSINE'>LYR</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ksy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ksy OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2ksy RCSB], [http://www.ebi.ac.uk/pdbsum/2ksy PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | == Evolutionary Conservation == | ||
| + | [[Image:Consurf_key_small.gif|200px|right]] | ||
| + | Check<jmol> | ||
| + | <jmolCheckbox> | ||
| + | <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ks/2ksy_consurf.spt"</scriptWhenChecked> | ||
| + | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | ||
| + | <text>to colour the structure by Evolutionary Conservation</text> | ||
| + | </jmolCheckbox> | ||
| + | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf]. | ||
| + | <div style="clear:both"></div> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Seven-helix membrane proteins represent a challenge for structural biology. Here we report the first NMR structure determination of a detergent-solubilized seven-helix transmembrane (7TM) protein, the phototaxis receptor sensory rhodopsin II (pSRII) from Natronomonas pharaonis, as a proof of principle. The overall quality of the structure ensemble is good (backbone r.m.s. deviation of 0.48 A) and agrees well with previously determined X-ray structures. Furthermore, measurements in more native-like small phospholipid bicelles indicate that the protein structure is the same as in detergent micelles, suggesting that environment-specific effects are minimal when using mild detergents. We use our case study as a platform to discuss the feasibility of similar solution NMR studies for other 7TM proteins, including members of the family of G protein-coupled receptors. | ||
| - | + | Structure determination of the seven-helix transmembrane receptor sensory rhodopsin II by solution NMR spectroscopy.,Gautier A, Mott HR, Bostock MJ, Kirkpatrick JP, Nietlispach D Nat Struct Mol Biol. 2010 Jun;17(6):768-74. Epub 2010 May 30. PMID:20512150<ref>PMID:20512150</ref> | |
| - | + | ||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
==See Also== | ==See Also== | ||
*[[Bacteriorhodopsin|Bacteriorhodopsin]] | *[[Bacteriorhodopsin|Bacteriorhodopsin]] | ||
*[[Rhodopsin|Rhodopsin]] | *[[Rhodopsin|Rhodopsin]] | ||
| - | + | == References == | |
| - | == | + | <references/> |
| - | < | + | __TOC__ |
| + | </StructureSection> | ||
[[Category: Natronomonas pharaonis]] | [[Category: Natronomonas pharaonis]] | ||
| - | [[Category: Bostock, M J | + | [[Category: Bostock, M J]] |
| - | [[Category: Gautier, A | + | [[Category: Gautier, A]] |
| - | [[Category: Kirkpatrick, J P | + | [[Category: Kirkpatrick, J P]] |
| - | [[Category: Mott, H R | + | [[Category: Mott, H R]] |
| - | [[Category: Nietlispach, D | + | [[Category: Nietlispach, D]] |
| - | + | ||
[[Category: Membrane protein]] | [[Category: Membrane protein]] | ||
[[Category: Nmr spectroscopy]] | [[Category: Nmr spectroscopy]] | ||
Revision as of 13:52, 17 December 2014
Solution nmr structure of sensory rhodopsin II
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