2l3j
From Proteopedia
(Difference between revisions)
| Line 1: | Line 1: | ||
| - | + | ==The solution structure of the ADAR2 dsRBM-RNA complex reveals a sequence-specific read out of the minor groove== | |
| - | + | <StructureSection load='2l3j' size='340' side='right' caption='[[2l3j]], [[NMR_Ensembles_of_Models | 19 NMR models]]' scene=''> | |
| - | + | == Structural highlights == | |
| - | + | <table><tr><td colspan='2'>[[2l3j]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2L3J OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2L3J FirstGlance]. <br> | |
| + | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Adarb1, Red1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10116 Rattus norvegicus])</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2l3j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2l3j OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2l3j RCSB], [http://www.ebi.ac.uk/pdbsum/2l3j PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Sequence-dependent recognition of dsDNA-binding proteins is well understood, yet sequence-specific recognition of dsRNA by proteins remains largely unknown, despite their importance in RNA maturation pathways. Adenosine deaminases that act on RNA (ADARs) recode genomic information by the site-selective deamination of adenosine. Here, we report the solution structure of the ADAR2 double-stranded RNA-binding motifs (dsRBMs) bound to a stem-loop pre-mRNA encoding the R/G editing site of GluR-2. The structure provides a molecular basis for how dsRBMs recognize the shape, and also more surprisingly, the sequence of the dsRNA. The unexpected direct readout of the RNA primary sequence by dsRBMs is achieved via the minor groove of the dsRNA and this recognition is critical for both editing and binding affinity at the R/G site of GluR-2. More generally, our findings suggest a solution to the sequence-specific paradox faced by many dsRBM-containing proteins that are involved in post-transcriptional regulation of gene expression. | ||
| - | + | The solution structure of the ADAR2 dsRBM-RNA complex reveals a sequence-specific readout of the minor groove.,Stefl R, Oberstrass FC, Hood JL, Jourdan M, Zimmermann M, Skrisovska L, Maris C, Peng L, Hofr C, Emeson RB, Allain FH Cell. 2010 Oct 15;143(2):225-37. PMID:20946981<ref>PMID:20946981</ref> | |
| - | + | ||
| - | == | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> |
| - | < | + | </div> |
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
[[Category: Rattus norvegicus]] | [[Category: Rattus norvegicus]] | ||
| - | [[Category: Allain, F H.T | + | [[Category: Allain, F H.T]] |
| - | [[Category: Oberstrass, F C | + | [[Category: Oberstrass, F C]] |
| - | [[Category: Stefl, R | + | [[Category: Stefl, R]] |
[[Category: Dsrbm]] | [[Category: Dsrbm]] | ||
[[Category: Dsrna recognition]] | [[Category: Dsrna recognition]] | ||
[[Category: Editing]] | [[Category: Editing]] | ||
[[Category: Hydrolase-rna complex]] | [[Category: Hydrolase-rna complex]] | ||
Revision as of 14:04, 17 December 2014
The solution structure of the ADAR2 dsRBM-RNA complex reveals a sequence-specific read out of the minor groove
| |||||||||||
