1c49
From Proteopedia
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| - | + | ==STRUCTURAL AND FUNCTIONAL DIFFERENCES OF TWO TOXINS FROM THE SCORPION PANDINUS IMPERATOR== | |
| - | + | <StructureSection load='1c49' size='340' side='right' caption='[[1c49]], [[NMR_Ensembles_of_Models | 18 NMR models]]' scene=''> | |
| - | + | == Structural highlights == | |
| + | <table><tr><td colspan='2'>[[1c49]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Pandinus_imperator Pandinus imperator]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1C49 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1C49 FirstGlance]. <br> | ||
| + | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1c49 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1c49 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1c49 RCSB], [http://www.ebi.ac.uk/pdbsum/1c49 PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The Pandinotoxins, PiTX-K alpha and PiTX-K beta, are members of the Charybdotoxin family of scorpion toxins that can be used to characterize K+ channels. PiTX-K alpha differs from PiTX-K beta, another peptide from Pandinus imperator, by one residue (P10E). When the two toxins are compared in a physiological assay, the affinity of PiTX-K beta for voltage-gated, rapidly inactivating K+ channels in dorsal root ganglia (DRG) neurons is 800-fold lower than that of PiTX-K alpha (K alpha-IC50 = 8.0 nM versus K beta-IC50 = 6,500 nM). To understand this difference, the three-dimensional structure of PiTX-K beta was determined by nuclear magnetic resonance (NMR) spectroscopy and compared to that of PiTX-K alpha. This comparison shows that structural differences between the two toxins occur at a residue that is critical for blocking K+ channels (K27) as well as at the site of the natural mutation (P10E). In PiTX-K beta, the negatively charged carboxylate oxygen of E10 can approach the positive charge of K27 and presumably reduces the net positive charge in this region of the toxin. This is likely the reason why PiTX-K beta binds K+ channels from DRG neurons with a much lower affinity than does PiTX-K alpha. | ||
| - | + | Structural and functional differences of two toxins from the scorpion Pandinus imperator.,Klenk KC, Tenenholz TC, Matteson DR, Rogowski RS, Blaustein MP, Weber DJ Proteins. 2000 Mar 1;38(4):441-9. PMID:10707030<ref>PMID:10707030</ref> | |
| - | + | ||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
==See Also== | ==See Also== | ||
*[[Potassium channel toxin|Potassium channel toxin]] | *[[Potassium channel toxin|Potassium channel toxin]] | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
[[Category: Pandinus imperator]] | [[Category: Pandinus imperator]] | ||
| - | [[Category: Blaustein, M P | + | [[Category: Blaustein, M P]] |
| - | [[Category: Klenk, K C | + | [[Category: Klenk, K C]] |
| - | [[Category: Matteson, D R | + | [[Category: Matteson, D R]] |
| - | [[Category: Rogowski, R S | + | [[Category: Rogowski, R S]] |
| - | [[Category: Tenenholz, T C | + | [[Category: Tenenholz, T C]] |
| - | [[Category: Weber, D J | + | [[Category: Weber, D J]] |
[[Category: Alpha-k toxin family]] | [[Category: Alpha-k toxin family]] | ||
[[Category: Neurotoxin]] | [[Category: Neurotoxin]] | ||
Revision as of 14:10, 17 December 2014
STRUCTURAL AND FUNCTIONAL DIFFERENCES OF TWO TOXINS FROM THE SCORPION PANDINUS IMPERATOR
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