1grz
From Proteopedia
(Difference between revisions)
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| - | + | ==A PREORGANIZED ACTIVE SITE IN THE CRYSTAL STRUCTURE OF THE TETRAHYMENA RIBOZYME== | |
| - | + | <StructureSection load='1grz' size='340' side='right' caption='[[1grz]], [[Resolution|resolution]] 5.00Å' scene=''> | |
| - | + | == Structural highlights == | |
| + | <table><tr><td colspan='2'>[[1grz]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Tetrahymena_thermophila Tetrahymena thermophila]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GRZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1GRZ FirstGlance]. <br> | ||
| + | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1grz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1grz OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1grz RCSB], [http://www.ebi.ac.uk/pdbsum/1grz PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Group I introns possess a single active site that catalyzes the two sequential reactions of self-splicing. An RNA comprising the two domains of the Tetrahymena thermophila group I intron catalytic core retains activity, and the 5.0 angstrom crystal structure of this 247-nucleotide ribozyme is now described. Close packing of the two domains forms a shallow cleft capable of binding the short helix that contains the 5' splice site. The helix that provides the binding site for the guanosine substrate deviates significantly from A-form geometry, providing a tight binding pocket. The binding pockets for both the 5' splice site helix and guanosine are formed and oriented in the absence of these substrates. Thus, this large ribozyme is largely preorganized for catalysis, much like a globular protein enzyme. | ||
| - | + | A preorganized active site in the crystal structure of the Tetrahymena ribozyme.,Golden BL, Gooding AR, Podell ER, Cech TR Science. 1998 Oct 9;282(5387):259-64. PMID:9841391<ref>PMID:9841391</ref> | |
| - | + | ||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
==See Also== | ==See Also== | ||
*[[Group I intron|Group I intron]] | *[[Group I intron|Group I intron]] | ||
| - | *[[Hemoglobin|Hemoglobin]] | + | *[[Hemoglobin 3D structures|Hemoglobin 3D structures]] |
*[[Ribozyme|Ribozyme]] | *[[Ribozyme|Ribozyme]] | ||
| - | + | == References == | |
| - | == | + | <references/> |
| - | < | + | __TOC__ |
| + | </StructureSection> | ||
[[Category: Tetrahymena thermophila]] | [[Category: Tetrahymena thermophila]] | ||
| - | [[Category: Cech, T R | + | [[Category: Cech, T R]] |
| - | [[Category: Golden, B L | + | [[Category: Golden, B L]] |
| - | [[Category: Gooding, A R | + | [[Category: Gooding, A R]] |
| - | [[Category: Podell, E R | + | [[Category: Podell, E R]] |
[[Category: Ribozyme]] | [[Category: Ribozyme]] | ||
[[Category: Ribozyme structure]] | [[Category: Ribozyme structure]] | ||
[[Category: Rrna group i intron]] | [[Category: Rrna group i intron]] | ||
Revision as of 14:14, 17 December 2014
A PREORGANIZED ACTIVE SITE IN THE CRYSTAL STRUCTURE OF THE TETRAHYMENA RIBOZYME
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