2kw8
From Proteopedia
(Difference between revisions)
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| - | + | ==Solution Structure of Bacillus anthracis Sortase A (SrtA) Transpeptidase== | |
| - | + | <StructureSection load='2kw8' size='340' side='right' caption='[[2kw8]], [[NMR_Ensembles_of_Models | 40 NMR models]]' scene=''> | |
| - | === | + | == Structural highlights == |
| - | + | <table><tr><td colspan='2'>[[2kw8]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_anthracis Bacillus anthracis]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KW8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2KW8 FirstGlance]. <br> | |
| + | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">BA_0688, GBAA0688, GBAA_0688 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1392 Bacillus anthracis])</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2kw8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2kw8 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2kw8 RCSB], [http://www.ebi.ac.uk/pdbsum/2kw8 PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | == Evolutionary Conservation == | ||
| + | [[Image:Consurf_key_small.gif|200px|right]] | ||
| + | Check<jmol> | ||
| + | <jmolCheckbox> | ||
| + | <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/kw/2kw8_consurf.spt"</scriptWhenChecked> | ||
| + | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | ||
| + | <text>to colour the structure by Evolutionary Conservation</text> | ||
| + | </jmolCheckbox> | ||
| + | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf]. | ||
| + | <div style="clear:both"></div> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The pathogen Bacillus anthracis uses the Sortase A (SrtA) enzyme to anchor proteins to its cell wall envelope during vegetative growth. To gain insight into the mechanism of protein attachment to the cell wall in B. anthracis we investigated the structure, backbone dynamics, and function of SrtA. The NMR structure of SrtA has been determined with a backbone coordinate precision of 0.40 +/- 0.07 A. SrtA possesses several novel features not previously observed in sortase enzymes including the presence of a structurally ordered amino terminus positioned within the active site and in contact with catalytically essential histidine residue (His(126)). We propose that this appendage, in combination with a unique flexible active site loop, mediates the recognition of lipid II, the second substrate to which proteins are attached during the anchoring reaction. pK(a) measurements indicate that His(126) is uncharged at physiological pH compatible with the enzyme operating through a "reverse protonation" mechanism. Interestingly, NMR relaxation measurements and the results of a model building study suggest that SrtA recognizes the LPXTG sorting signal through a lock-in-key mechanism in contrast to the prototypical SrtA enzyme from Staphylococcus aureus. | ||
| - | + | The Sortase A enzyme that attaches proteins to the cell wall of Bacillus anthracis contains an unusual active site architecture.,Weiner EM, Robson S, Marohn M, Clubb RT J Biol Chem. 2010 Jul 23;285(30):23433-43. Epub 2010 May 19. PMID:20489200<ref>PMID:20489200</ref> | |
| - | + | ||
| - | == | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> |
| - | < | + | </div> |
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
[[Category: Bacillus anthracis]] | [[Category: Bacillus anthracis]] | ||
| - | [[Category: Clubb, R T | + | [[Category: Clubb, R T]] |
| - | [[Category: Marohn, M | + | [[Category: Marohn, M]] |
| - | [[Category: Robson, S A | + | [[Category: Robson, S A]] |
| - | [[Category: Weiner, E M | + | [[Category: Weiner, E M]] |
[[Category: Protein binding]] | [[Category: Protein binding]] | ||
[[Category: Sortase]] | [[Category: Sortase]] | ||
[[Category: Srta]] | [[Category: Srta]] | ||
[[Category: Transpeptidase]] | [[Category: Transpeptidase]] | ||
Revision as of 14:15, 17 December 2014
Solution Structure of Bacillus anthracis Sortase A (SrtA) Transpeptidase
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