1hp2
From Proteopedia
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| - | + | ==SOLUTION STRUCTURE OF A TOXIN FROM THE SCORPION TITYUS SERRULATUS (TSTX-K ALPHA) DETERMINED BY NMR.== | |
| - | + | <StructureSection load='1hp2' size='340' side='right' caption='[[1hp2]], [[NMR_Ensembles_of_Models | 30 NMR models]]' scene=''> | |
| - | + | == Structural highlights == | |
| + | <table><tr><td colspan='2'>[[1hp2]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Tityus_serrulatus Tityus serrulatus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HP2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1HP2 FirstGlance]. <br> | ||
| + | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1hp2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1hp2 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1hp2 RCSB], [http://www.ebi.ac.uk/pdbsum/1hp2 PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | A toxin from the scorpion Tityus serrulatus (TsTX-Kalpha) blocks native squid K(+) channels and their cloned counterpart, sqKv1A, at pH 8 ((native)K(d) approximately 20 nM; (sqKv1A)K(d) approximately 10 nM). In both cases, decreasing the pH below 7.0 significantly diminishes the TsTX-Kalpha effect (pK = 6.6). In the cloned squid channel, the pH dependence of the block is abolished by a single point mutation (H351G), and no change in toxin affinity was observed at higher pH values (pH > or =8.0). To further investigate the TsTX-Kalpha-sqKv1A interaction, the three-dimensional structure of TsTX-Kalpha was determined in solution by NMR spectroscopy, and a model of the TsTX-Kalpha-sqKv1A complex was generated. As found for other alpha-K toxins such as charybdotoxin (CTX), site-directed mutagenesis at toxin residue K27 (K27A, K27R, and K27E) significantly reduced the toxin's affinity for sqKv1A channels. This is consistent with the TsTX-Kalpha-sqKv1A model reported here, which has K27 of the toxin inserted into the ion conduction pathway of the K(+) channel. This toxin-channel model also illustrates a possible mechanism for the pH-dependent block whereby lysine residues from TsTX-Kalpha (K6 and K23) are repelled by protonated H351 on sqKv1A at low pH. | ||
| - | + | Interaction of a toxin from the scorpion Tityus serrulatus with a cloned K+ channel from squid (sqKv1A).,Ellis KC, Tenenholz TC, Jerng H, Hayhurst M, Dudlak CS, Gilly WF, Blaustein MP, Weber DJ Biochemistry. 2001 May 22;40(20):5942-53. PMID:11352729<ref>PMID:11352729</ref> | |
| - | + | ||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
==See Also== | ==See Also== | ||
*[[Potassium channel toxin|Potassium channel toxin]] | *[[Potassium channel toxin|Potassium channel toxin]] | ||
| - | + | == References == | |
| - | == | + | <references/> |
| - | < | + | __TOC__ |
| + | </StructureSection> | ||
[[Category: Tityus serrulatus]] | [[Category: Tityus serrulatus]] | ||
| - | [[Category: Blaustein, M P | + | [[Category: Blaustein, M P]] |
| - | [[Category: Ellis, K C | + | [[Category: Ellis, K C]] |
| - | [[Category: Gilly, W F | + | [[Category: Gilly, W F]] |
| - | [[Category: Tenenholz, T C | + | [[Category: Tenenholz, T C]] |
| - | [[Category: Weber, D J | + | [[Category: Weber, D J]] |
[[Category: Alpha-k toxin]] | [[Category: Alpha-k toxin]] | ||
[[Category: K+ channel]] | [[Category: K+ channel]] | ||
[[Category: Scorpion toxin]] | [[Category: Scorpion toxin]] | ||
[[Category: Toxin]] | [[Category: Toxin]] | ||
Revision as of 14:15, 17 December 2014
SOLUTION STRUCTURE OF A TOXIN FROM THE SCORPION TITYUS SERRULATUS (TSTX-K ALPHA) DETERMINED BY NMR.
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