1p7i
From Proteopedia
| Line 1: | Line 1: | ||
| - | [[Image:1p7i.jpg|left|200px]] | + | [[Image:1p7i.jpg|left|200px]] |
| - | + | ||
| - | '''CRYSTAL STRUCTURE OF ENGRAILED HOMEODOMAIN MUTANT K52A''' | + | {{Structure |
| + | |PDB= 1p7i |SIZE=350|CAPTION= <scene name='initialview01'>1p7i</scene>, resolution 2.10Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=NHE:2-[N-CYCLOHEXYLAMINO]ETHANE SULFONIC ACID'>NHE</scene> | ||
| + | |ACTIVITY= | ||
| + | |GENE= EN ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=7227 Drosophila melanogaster]) | ||
| + | }} | ||
| + | |||
| + | '''CRYSTAL STRUCTURE OF ENGRAILED HOMEODOMAIN MUTANT K52A''' | ||
| + | |||
==Overview== | ==Overview== | ||
| Line 7: | Line 16: | ||
==About this Structure== | ==About this Structure== | ||
| - | 1P7I is a [ | + | 1P7I is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1P7I OCA]. |
==Reference== | ==Reference== | ||
| - | Crystal structures of engrailed homeodomain mutants: implications for stability and dynamics., Stollar EJ, Mayor U, Lovell SC, Federici L, Freund SM, Fersht AR, Luisi BF, J Biol Chem. 2003 Oct 31;278(44):43699-708. Epub 2003 Aug 15. PMID:[http:// | + | Crystal structures of engrailed homeodomain mutants: implications for stability and dynamics., Stollar EJ, Mayor U, Lovell SC, Federici L, Freund SM, Fersht AR, Luisi BF, J Biol Chem. 2003 Oct 31;278(44):43699-708. Epub 2003 Aug 15. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12923178 12923178] |
[[Category: Drosophila melanogaster]] | [[Category: Drosophila melanogaster]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| Line 23: | Line 32: | ||
[[Category: dna binding protein]] | [[Category: dna binding protein]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 13:20:54 2008'' |
Revision as of 11:20, 20 March 2008
| |||||||
| , resolution 2.10Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | |||||||
| Gene: | EN (Drosophila melanogaster) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
CRYSTAL STRUCTURE OF ENGRAILED HOMEODOMAIN MUTANT K52A
Overview
We report the crystal structures and biophysical characterization of two stabilized mutants of the Drosophila Engrailed homeodomain that have been engineered to minimize electrostatic repulsion. Four independent copies of each mutant occupy the crystal lattice, and comparison of these structures illustrates variation that can be partly ascribed to networks of correlated conformational adjustments. Central to one network is leucine 26 (Leu26), which occupies alternatively two side chain rotameric conformations (-gauche and trans) and different positions within the hydrophobic core. Similar sets of conformational substates are observed in other Engrailed structures and in another homeodomain. The pattern of structural adjustments can account for NMR relaxation data and sequence co-variation networks in the wider homeodomain family. It may also explain the dysfunction associated with a P26L mutation in the human ARX homeodomain protein. Finally, we observe a novel dipolar interaction between a conserved tryptophan and a water molecule positioned along the normal to the indole ring. This interaction may explain the distinctive fluorescent properties of the homeodomain family.
About this Structure
1P7I is a Single protein structure of sequence from Drosophila melanogaster. Full crystallographic information is available from OCA.
Reference
Crystal structures of engrailed homeodomain mutants: implications for stability and dynamics., Stollar EJ, Mayor U, Lovell SC, Federici L, Freund SM, Fersht AR, Luisi BF, J Biol Chem. 2003 Oct 31;278(44):43699-708. Epub 2003 Aug 15. PMID:12923178
Page seeded by OCA on Thu Mar 20 13:20:54 2008
