1gid
From Proteopedia
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| - | + | ==CRYSTAL STRUCTURE OF A GROUP I RIBOZYME DOMAIN: PRINCIPLES OF RNA PACKING== | |
| - | + | <StructureSection load='1gid' size='340' side='right' caption='[[1gid]], [[Resolution|resolution]] 2.50Å' scene=''> | |
| - | + | == Structural highlights == | |
| + | <table><tr><td colspan='2'>[[1gid]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GID OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1GID FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NCO:COBALT+HEXAMMINE(III)'>NCO</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1gid FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1gid OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1gid RCSB], [http://www.ebi.ac.uk/pdbsum/1gid PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Group I self-splicing introns catalyze their own excision from precursor RNAs by way of a two-step transesterification reaction. The catalytic core of these ribozymes is formed by two structural domains. The 2.8-angstrom crystal structure of one of these, the P4-P6 domain of the Tetrahymena thermophila intron, is described. In the 160-nucleotide domain, a sharp bend allows stacked helices of the conserved core to pack alongside helices of an adjacent region. Two specific long-range interactions clamp the two halves of the domain together: a two-Mg2+-coordinated adenosine-rich corkscrew plugs into the minor groove of a helix, and a GAAA hairpin loop binds to a conserved 11-nucleotide internal loop. Metal- and ribose-mediated backbone contacts further stabilize the close side-by-side helical packing. The structure indicates the extent of RNA packing required for the function of large ribozymes, the spliceosome, and the ribosome. | ||
| - | + | Crystal structure of a group I ribozyme domain: principles of RNA packing.,Cate JH, Gooding AR, Podell E, Zhou K, Golden BL, Kundrot CE, Cech TR, Doudna JA Science. 1996 Sep 20;273(5282):1678-85. PMID:8781224<ref>PMID:8781224</ref> | |
| - | + | ||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
==See Also== | ==See Also== | ||
*[[Group I intron|Group I intron]] | *[[Group I intron|Group I intron]] | ||
*[[Ribozyme|Ribozyme]] | *[[Ribozyme|Ribozyme]] | ||
| - | + | == References == | |
| - | == | + | <references/> |
| - | < | + | __TOC__ |
| + | </StructureSection> | ||
[[Category: Synthetic construct]] | [[Category: Synthetic construct]] | ||
| - | [[Category: Cate, J H | + | [[Category: Cate, J H]] |
| - | [[Category: Cech, T R | + | [[Category: Cech, T R]] |
| - | [[Category: Doudna, J A | + | [[Category: Doudna, J A]] |
| - | [[Category: Golden, B L | + | [[Category: Golden, B L]] |
| - | [[Category: Gooding, A R | + | [[Category: Gooding, A R]] |
| - | [[Category: Kundrot, C E | + | [[Category: Kundrot, C E]] |
| - | [[Category: Podell, E | + | [[Category: Podell, E]] |
| - | [[Category: Zhou, K | + | [[Category: Zhou, K]] |
[[Category: P4-p6 ribozyme domain of the tetrahymena group i intron]] | [[Category: P4-p6 ribozyme domain of the tetrahymena group i intron]] | ||
[[Category: Ribozyme]] | [[Category: Ribozyme]] | ||
[[Category: Rna]] | [[Category: Rna]] | ||
Revision as of 14:16, 17 December 2014
CRYSTAL STRUCTURE OF A GROUP I RIBOZYME DOMAIN: PRINCIPLES OF RNA PACKING
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