1p8k
From Proteopedia
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| - | [[Image:1p8k.gif|left|200px]] | + | [[Image:1p8k.gif|left|200px]] |
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| - | '''The structure and DNA recognition of a bifunctional homing endonuclease and group I intron splicing factor''' | + | {{Structure |
| + | |PDB= 1p8k |SIZE=350|CAPTION= <scene name='initialview01'>1p8k</scene>, resolution 2.6Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=MG:MAGNESIUM ION'>MG</scene> | ||
| + | |ACTIVITY= | ||
| + | |GENE= Aspergillus nidulans ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=162425 Emericella nidulans]) | ||
| + | }} | ||
| + | |||
| + | '''The structure and DNA recognition of a bifunctional homing endonuclease and group I intron splicing factor''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1P8K is a [ | + | 1P8K is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Emericella_nidulans Emericella nidulans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1P8K OCA]. |
==Reference== | ==Reference== | ||
| - | Structural and biochemical analyses of DNA and RNA binding by a bifunctional homing endonuclease and group I intron splicing factor., Bolduc JM, Spiegel PC, Chatterjee P, Brady KL, Downing ME, Caprara MG, Waring RB, Stoddard BL, Genes Dev. 2003 Dec 1;17(23):2875-88. Epub 2003 Nov 21. PMID:[http:// | + | Structural and biochemical analyses of DNA and RNA binding by a bifunctional homing endonuclease and group I intron splicing factor., Bolduc JM, Spiegel PC, Chatterjee P, Brady KL, Downing ME, Caprara MG, Waring RB, Stoddard BL, Genes Dev. 2003 Dec 1;17(23):2875-88. Epub 2003 Nov 21. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/14633971 14633971] |
[[Category: Emericella nidulans]] | [[Category: Emericella nidulans]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: hydrolase/dna]] | [[Category: hydrolase/dna]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 13:21:20 2008'' |
Revision as of 11:21, 20 March 2008
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| , resolution 2.6Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | |||||||
| Gene: | Aspergillus nidulans (Emericella nidulans) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
The structure and DNA recognition of a bifunctional homing endonuclease and group I intron splicing factor
Overview
We determined the crystal structure of a bifunctional group I intron splicing factor and homing endonuclease, termed the I-AniI maturase, in complex with its DNA target at 2.6 A resolution. The structure demonstrates the remarkable structural conservation of the beta-sheet DNA-binding motif between highly divergent enzyme subfamilies. DNA recognition by I-AniI was further studied using nucleoside deletion and DMS modification interference analyses. Correlation of these results with the crystal structure provides information on the relative importance of individual nucleotide contacts for DNA recognition. Alignment and modeling of two homologous maturases reveals conserved basic surface residues, distant from the DNA-binding surface, that might be involved in RNA binding. A point mutation that introduces a single negative charge in this region uncouples the maturase and endonuclease functions of the protein, inhibiting RNA binding and splicing while maintaining DNA binding and cleavage.
About this Structure
1P8K is a Single protein structure of sequence from Emericella nidulans. Full crystallographic information is available from OCA.
Reference
Structural and biochemical analyses of DNA and RNA binding by a bifunctional homing endonuclease and group I intron splicing factor., Bolduc JM, Spiegel PC, Chatterjee P, Brady KL, Downing ME, Caprara MG, Waring RB, Stoddard BL, Genes Dev. 2003 Dec 1;17(23):2875-88. Epub 2003 Nov 21. PMID:14633971
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