This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
3hx4
From Proteopedia
(Difference between revisions)
m (Protected "3hx4" [edit=sysop:move=sysop]) |
|||
| Line 1: | Line 1: | ||
| - | + | ==Crystal structure of CDPK1 of Toxoplasma gondii, TGME49_101440, in presence of calcium== | |
| - | + | <StructureSection load='3hx4' size='340' side='right' caption='[[3hx4]], [[Resolution|resolution]] 1.95Å' scene=''> | |
| - | + | == Structural highlights == | |
| + | <table><tr><td colspan='2'>[[3hx4]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Toxoplasma_gondii Toxoplasma gondii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3HX4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3HX4 FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ANP:PHOSPHOAMINOPHOSPHONIC+ACID-ADENYLATE+ESTER'>ANP</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | ||
| + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">TGME49_101440, CDPK1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5811 Toxoplasma gondii])</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3hx4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3hx4 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3hx4 RCSB], [http://www.ebi.ac.uk/pdbsum/3hx4 PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | == Evolutionary Conservation == | ||
| + | [[Image:Consurf_key_small.gif|200px|right]] | ||
| + | Check<jmol> | ||
| + | <jmolCheckbox> | ||
| + | <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/hx/3hx4_consurf.spt"</scriptWhenChecked> | ||
| + | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | ||
| + | <text>to colour the structure by Evolutionary Conservation</text> | ||
| + | </jmolCheckbox> | ||
| + | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf]. | ||
| + | <div style="clear:both"></div> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Calcium-dependent protein kinases (CDPKs) have pivotal roles in the calcium-signaling pathway in plants, ciliates and apicomplexan parasites and comprise a calmodulin-dependent kinase (CaMK)-like kinase domain regulated by a calcium-binding domain in the C terminus. To understand this intramolecular mechanism of activation, we solved the structures of the autoinhibited (apo) and activated (calcium-bound) conformations of CDPKs from the apicomplexan parasites Toxoplasma gondii and Cryptosporidium parvum. In the apo form, the C-terminal CDPK activation domain (CAD) resembles a calmodulin protein with an unexpected long helix in the N terminus that inhibits the kinase domain in the same manner as CaMKII. Calcium binding triggers the reorganization of the CAD into a highly intricate fold, leading to its relocation around the base of the kinase domain to a site remote from the substrate binding site. This large conformational change constitutes a distinct mechanism in calcium signal-transduction pathways. | ||
| - | + | Structures of apicomplexan calcium-dependent protein kinases reveal mechanism of activation by calcium.,Wernimont AK, Artz JD, Finerty P Jr, Lin YH, Amani M, Allali-Hassani A, Senisterra G, Vedadi M, Tempel W, Mackenzie F, Chau I, Lourido S, Sibley LD, Hui R Nat Struct Mol Biol. 2010 May;17(5):596-601. Epub 2010 May 2. PMID:20436473<ref>PMID:20436473</ref> | |
| - | + | ||
| - | == | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> |
| - | + | </div> | |
| + | |||
| + | ==See Also== | ||
| + | *[[Calcium-dependent protein kinase|Calcium-dependent protein kinase]] | ||
| + | *[[EF hand|EF hand]] | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
[[Category: Toxoplasma gondii]] | [[Category: Toxoplasma gondii]] | ||
| - | [[Category: Arrowsmith, C H | + | [[Category: Arrowsmith, C H]] |
| - | [[Category: Artz, J D | + | [[Category: Artz, J D]] |
| - | [[Category: Bochkarev, A | + | [[Category: Bochkarev, A]] |
| - | [[Category: Bountra, C | + | [[Category: Bountra, C]] |
| - | [[Category: Edwards, A M | + | [[Category: Edwards, A M]] |
| - | [[Category: Finnerty, P | + | [[Category: Finnerty, P]] |
| - | [[Category: Hassani, A A | + | [[Category: Hassani, A A]] |
| - | [[Category: He, H | + | [[Category: He, H]] |
| - | [[Category: Hui, R | + | [[Category: Hui, R]] |
| - | [[Category: Lin, Y H | + | [[Category: Lin, Y H]] |
| - | [[Category: Lourido, S | + | [[Category: Lourido, S]] |
| - | [[Category: MacKenzie, F | + | [[Category: MacKenzie, F]] |
| - | [[Category: | + | [[Category: Structural genomic]] |
| - | [[Category: Sibley, D L | + | [[Category: Sibley, D L]] |
| - | [[Category: Sinestera, G | + | [[Category: Sinestera, G]] |
| - | [[Category: Vedadi, M | + | [[Category: Vedadi, M]] |
| - | [[Category: Wasney, G | + | [[Category: Wasney, G]] |
| - | [[Category: Weigelt, J | + | [[Category: Weigelt, J]] |
| - | [[Category: Wernimont, A K | + | [[Category: Wernimont, A K]] |
| - | [[Category: Xiao, T | + | [[Category: Xiao, T]] |
[[Category: Atp-binding]] | [[Category: Atp-binding]] | ||
[[Category: Cdpk]] | [[Category: Cdpk]] | ||
| Line 36: | Line 61: | ||
[[Category: Serine/threonine-protein kinase]] | [[Category: Serine/threonine-protein kinase]] | ||
[[Category: Sgc]] | [[Category: Sgc]] | ||
| - | [[Category: Structural genomic]] | ||
| - | [[Category: Structural genomics consortium]] | ||
[[Category: Toxoplasma]] | [[Category: Toxoplasma]] | ||
[[Category: Transferase]] | [[Category: Transferase]] | ||
Revision as of 06:13, 18 December 2014
Crystal structure of CDPK1 of Toxoplasma gondii, TGME49_101440, in presence of calcium
| |||||||||||
Categories: Toxoplasma gondii | Arrowsmith, C H | Artz, J D | Bochkarev, A | Bountra, C | Edwards, A M | Finnerty, P | Hassani, A A | He, H | Hui, R | Lin, Y H | Lourido, S | MacKenzie, F | Structural genomic | Sibley, D L | Sinestera, G | Vedadi, M | Wasney, G | Weigelt, J | Wernimont, A K | Xiao, T | Atp-binding | Cdpk | Kinase | Nucleotide-binding | Protist | Serine/threonine-protein kinase | Sgc | Toxoplasma | Transferase
