3hrv

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{{STRUCTURE_3hrv| PDB=3hrv | SCENE= }}
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==Crystal structure of TcpA, a Type IV pilin from Vibrio cholerae El Tor biotype==
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===Crystal structure of TcpA, a Type IV pilin from Vibrio cholerae El Tor biotype===
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<StructureSection load='3hrv' size='340' side='right' caption='[[3hrv]], [[Resolution|resolution]] 1.50&Aring;' scene=''>
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{{ABSTRACT_PUBMED_20545841}}
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== Structural highlights ==
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<table><tr><td colspan='2'>[[3hrv]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Vibrio_cholerae Vibrio cholerae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3HRV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3HRV FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1oqv|1oqv]]</td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">tcpA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=666 Vibrio cholerae])</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3hrv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3hrv OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3hrv RCSB], [http://www.ebi.ac.uk/pdbsum/3hrv PDBsum]</span></td></tr>
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</table>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/hr/3hrv_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Type IV pili (T4P) are critical to virulence for Vibrio cholerae and other bacterial pathogens. Among their diverse functions, T4P mediate microcolony formation, which protects the bacteria from host defences and concentrates secreted toxins. The T4P of the two V. cholerae O1 disease biotypes, classical and El Tor, share 81% identity in their TcpA subunits, yet these filaments differ in their interaction patterns as assessed by electron microscopy. To understand the molecular basis for pilus-mediated microcolony formation, we solved a 1.5 A resolution crystal structure of N-terminally truncated El Tor TcpA and compared it with that of classical TcpA. Residues that differ between the two pilins are located on surface-exposed regions of the TcpA subunits. By iteratively changing these non-conserved amino acids in classical TcpA to their respective residues in El Tor TcpA, we identified residues that profoundly affect pilus:pilus interaction patterns and bacterial aggregation. These residues lie on either the protruding d-region of the TcpA subunit or in a cavity between pilin subunits in the pilus filament. Our results support a model whereby pili interact via intercalation of surface protrusions on one filament into depressions between subunits on adjacent filaments as a means to hold V. cholerae cells together in microcolonies.
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==About this Structure==
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Vibrio cholerae El Tor TcpA crystal structure and mechanism for pilus-mediated microcolony formation.,Lim MS, Ng D, Zong Z, Arvai AS, Taylor RK, Tainer JA, Craig L Mol Microbiol. 2010 Aug;77(3):755-70. Epub 2010 Jun 1. PMID:20545841<ref>PMID:20545841</ref>
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[[3hrv]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Vibrio_cholerae Vibrio cholerae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3HRV OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<ref group="xtra">PMID:020545841</ref><references group="xtra"/>
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</div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
[[Category: Vibrio cholerae]]
[[Category: Vibrio cholerae]]
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[[Category: Arvai, A S.]]
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[[Category: Arvai, A S]]
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[[Category: Craig, L.]]
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[[Category: Craig, L]]
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[[Category: Tainer, J A.]]
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[[Category: Tainer, J A]]
[[Category: Cell adhesion]]
[[Category: Cell adhesion]]
[[Category: Colonization]]
[[Category: Colonization]]
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[[Category: Toxin-coregulated pilus]]
[[Category: Toxin-coregulated pilus]]
[[Category: Type iv pili]]
[[Category: Type iv pili]]
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[[Category: Vibrio cholerae]]
 
[[Category: Virulence factor]]
[[Category: Virulence factor]]

Revision as of 06:14, 18 December 2014

Crystal structure of TcpA, a Type IV pilin from Vibrio cholerae El Tor biotype

3hrv, resolution 1.50Å

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