3ifi

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{{STRUCTURE_3ifi| PDB=3ifi | SCENE= }}
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==2'-SeMe-dG modified octamer DNA==
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===2'-SeMe-dG modified octamer DNA===
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<StructureSection load='3ifi' size='340' side='right' caption='[[3ifi]], [[Resolution|resolution]] 1.20&Aring;' scene=''>
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{{ABSTRACT_PUBMED_20047333}}
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== Structural highlights ==
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<table><tr><td colspan='2'>[[3ifi]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3IFI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3IFI FirstGlance]. <br>
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</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=XUG:2-SE-METHYL-2-SELENOGUANOSINE+5-(DIHYDROGEN+PHOSPHATE)'>XUG</scene></td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1dns|1dns]], [[3iff|3iff]]</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ifi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ifi OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3ifi RCSB], [http://www.ebi.ac.uk/pdbsum/3ifi PDBsum]</span></td></tr>
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</table>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Selenium modification of nucleic acids is of great importance in X-ray crystal structure determination and functional study of nucleic acids. Herein, we describe a convenient synthesis of a new building block, the 2'-SeMe-modified guanosine (G(Se)) phosphoramidite, and report the first incorporation of the 2'-Se-G moiety into DNA. The X-ray crystal structure of the 2'-Se-modified octamer DNA (5'-GTG(Se)TACAC-3') was determined at a resolution of 1.20 A. We also found that the 2'-Se modification points to the minor groove and that the modified and native structures are virtually identical. Furthermore, we observed that the 2'-Se-G modification can significantly facilitate the crystal growth with respect to the corresponding native DNA.
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==About this Structure==
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Synthesis and crystal structure of 2'-se-modified guanosine containing DNA.,Salon J, Sheng J, Gan J, Huang Z J Org Chem. 2010 Feb 5;75(3):637-41. PMID:20047333<ref>PMID:20047333</ref>
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[[3ifi]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3IFI OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<ref group="xtra">PMID:020047333</ref><references group="xtra"/>
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</div>
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[[Category: Gan, J.]]
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== References ==
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[[Category: Huang, Z.]]
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<references/>
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[[Category: Salon, J.]]
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__TOC__
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[[Category: Sheng, J.]]
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</StructureSection>
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[[Category: Gan, J]]
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[[Category: Huang, Z]]
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[[Category: Salon, J]]
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[[Category: Sheng, J]]
[[Category: Dna]]
[[Category: Dna]]
[[Category: Guanosine]]
[[Category: Guanosine]]
[[Category: Nucleic acid]]
[[Category: Nucleic acid]]
[[Category: Selenium]]
[[Category: Selenium]]

Revision as of 06:20, 18 December 2014

2'-SeMe-dG modified octamer DNA

3ifi, resolution 1.20Å

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