1pag

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[[Image:1pag.jpg|left|200px]]<br /><applet load="1pag" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:1pag.jpg|left|200px]]
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caption="1pag, resolution 2.8&Aring;" />
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'''THE 2.5 ANGSTROMS STRUCTURE OF POKEWEED ANTIVIRAL PROTEIN'''<br />
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{{Structure
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|PDB= 1pag |SIZE=350|CAPTION= <scene name='initialview01'>1pag</scene>, resolution 2.8&Aring;
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|SITE=
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|LIGAND= <scene name='pdbligand=FMP:FORMYCIN-5'-MONOPHOSPHATE'>FMP</scene>
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|ACTIVITY=
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|GENE=
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}}
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'''THE 2.5 ANGSTROMS STRUCTURE OF POKEWEED ANTIVIRAL PROTEIN'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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1PAG is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Phytolacca_americana Phytolacca americana] with <scene name='pdbligand=FMP:'>FMP</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PAG OCA].
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1PAG is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Phytolacca_americana Phytolacca americana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PAG OCA].
==Reference==
==Reference==
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The 2.5 A structure of pokeweed antiviral protein., Monzingo AF, Collins EJ, Ernst SR, Irvin JD, Robertus JD, J Mol Biol. 1993 Oct 20;233(4):705-15. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=8411176 8411176]
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The 2.5 A structure of pokeweed antiviral protein., Monzingo AF, Collins EJ, Ernst SR, Irvin JD, Robertus JD, J Mol Biol. 1993 Oct 20;233(4):705-15. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/8411176 8411176]
[[Category: Phytolacca americana]]
[[Category: Phytolacca americana]]
[[Category: Single protein]]
[[Category: Single protein]]
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[[Category: protein synthesis inhibitor]]
[[Category: protein synthesis inhibitor]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:26:59 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 13:22:01 2008''

Revision as of 11:22, 20 March 2008

Image:1pag.jpg


PDB ID 1pag

Drag the structure with the mouse to rotate
, resolution 2.8Å
Ligands:
Coordinates: save as pdb, mmCIF, xml



THE 2.5 ANGSTROMS STRUCTURE OF POKEWEED ANTIVIRAL PROTEIN


Overview

The pokeweed antiviral protein (PAP), isolated from the leaves of Phytolacca americana, is one of a family of plant and bacterial ribosome-inhibiting proteins (RIPs) which act as specific N-glycosidases on rRNA. Here we report the three-dimensional structure of PAP determined to 2.5 A resolution by X-ray crystallography. After 14 rounds of refinement, the R factor is 0.17 for 5.0 to 2.5 A data. The protein is homologous with the A chain of ricin and exhibits a very similar folding pattern. The positions of key active site residues are also similar. We also report the 2.8 A structure of PAP complexed with a substrate analog, formycin 5'-monophosphate. As seen previously in ricin, the formycin ring is stacked between invariant tyrosines 72 and 123. Arg179 bonds to N-3 which is thought to be important in catalysis.

About this Structure

1PAG is a Single protein structure of sequence from Phytolacca americana. Full crystallographic information is available from OCA.

Reference

The 2.5 A structure of pokeweed antiviral protein., Monzingo AF, Collins EJ, Ernst SR, Irvin JD, Robertus JD, J Mol Biol. 1993 Oct 20;233(4):705-15. PMID:8411176

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