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3gqb

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{{STRUCTURE_3gqb| PDB=3gqb | SCENE= }}
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==Crystal Structure of the A3B3 complex from V-ATPase==
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===Crystal Structure of the A3B3 complex from V-ATPase===
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<StructureSection load='3gqb' size='340' side='right' caption='[[3gqb]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
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{{ABSTRACT_PUBMED_19893485}}
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== Structural highlights ==
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<table><tr><td colspan='2'>[[3gqb]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Thermus_thermophilus_hb8 Thermus thermophilus hb8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3GQB OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3GQB FirstGlance]. <br>
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</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">atpA, TTHA1273 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=300852 Thermus thermophilus HB8]), atpB, TTHA1272 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=300852 Thermus thermophilus HB8])</td></tr>
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<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/H(+)-transporting_two-sector_ATPase H(+)-transporting two-sector ATPase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.3.14 3.6.3.14] </span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3gqb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3gqb OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3gqb RCSB], [http://www.ebi.ac.uk/pdbsum/3gqb PDBsum]</span></td></tr>
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</table>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/gq/3gqb_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Vacuolar-type ATPases (V-ATPases) exist in various cellular membranes of many organisms to regulate physiological processes by controlling the acidic environment. Here, we have determined the crystal structure of the A(3)B(3) subcomplex of V-ATPase at 2.8 A resolution. The overall construction of the A(3)B(3) subcomplex is significantly different from that of the alpha(3)beta(3) sub-domain in F(o)F(1)-ATP synthase, because of the presence of a protruding 'bulge' domain feature in the catalytic A subunits. The A(3)B(3) subcomplex structure provides the first molecular insight at the catalytic and non-catalytic interfaces, which was not possible in the structures of the separate subunits alone. Specifically, in the non-catalytic interface, the B subunit seems to be incapable of binding ATP, which is a marked difference from the situation indicated by the structure of the F(o)F(1)-ATP synthase. In the catalytic interface, our mutational analysis, on the basis of the A(3)B(3) structure, has highlighted the presence of a cluster composed of key hydrophobic residues, which are essential for ATP hydrolysis by V-ATPases.
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==About this Structure==
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Crystal structure of A3B3 complex of V-ATPase from Thermus thermophilus.,Maher MJ, Akimoto S, Iwata M, Nagata K, Hori Y, Yoshida M, Yokoyama S, Iwata S, Yokoyama K EMBO J. 2009 Dec 2;28(23):3771-9. Epub 2009 Nov 5. PMID:19893485<ref>PMID:19893485</ref>
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[[3gqb]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Thermus_thermophilus_hb8 Thermus thermophilus hb8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3GQB OCA].
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
==See Also==
==See Also==
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*[[ATP synthase|ATP synthase]]
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*[[ATPase|ATPase]]
*[[V-ATPase|V-ATPase]]
*[[V-ATPase|V-ATPase]]
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== References ==
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==Reference==
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<references/>
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<ref group="xtra">PMID:019893485</ref><references group="xtra"/>
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__TOC__
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</StructureSection>
[[Category: Thermus thermophilus hb8]]
[[Category: Thermus thermophilus hb8]]
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[[Category: Akimoto, S.]]
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[[Category: Akimoto, S]]
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[[Category: Hori, Y.]]
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[[Category: Hori, Y]]
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[[Category: Iwata, M.]]
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[[Category: Iwata, M]]
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[[Category: Iwata, S.]]
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[[Category: Iwata, S]]
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[[Category: Meher, M.]]
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[[Category: Meher, M]]
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[[Category: Nagata, K.]]
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[[Category: Nagata, K]]
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[[Category: Yokoyama, K.]]
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[[Category: Yokoyama, K]]
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[[Category: Yokoyama, S.]]
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[[Category: Yokoyama, S]]
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[[Category: Yoshida, M.]]
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[[Category: Yoshida, M]]
[[Category: A3b3]]
[[Category: A3b3]]
[[Category: Atp synthesis]]
[[Category: Atp synthesis]]

Revision as of 06:24, 18 December 2014

Crystal Structure of the A3B3 complex from V-ATPase

3gqb, resolution 2.80Å

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