3k3f
From Proteopedia
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| - | + | ==Crystal Structure of the Urea Transporter from Desulfovibrio Vulgaris== | |
| - | === | + | <StructureSection load='3k3f' size='340' side='right' caption='[[3k3f]], [[Resolution|resolution]] 2.30Å' scene=''> |
| - | + | == Structural highlights == | |
| + | <table><tr><td colspan='2'>[[3k3f]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Desulfovibrio_vulgaris Desulfovibrio vulgaris]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3K3F OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3K3F FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=AU:GOLD+ION'>AU</scene></td></tr> | ||
| + | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3k3g|3k3g]]</td></tr> | ||
| + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">DVU_1160 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=881 Desulfovibrio vulgaris])</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3k3f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3k3f OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3k3f RCSB], [http://www.ebi.ac.uk/pdbsum/3k3f PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | == Evolutionary Conservation == | ||
| + | [[Image:Consurf_key_small.gif|200px|right]] | ||
| + | Check<jmol> | ||
| + | <jmolCheckbox> | ||
| + | <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/k3/3k3f_consurf.spt"</scriptWhenChecked> | ||
| + | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | ||
| + | <text>to colour the structure by Evolutionary Conservation</text> | ||
| + | </jmolCheckbox> | ||
| + | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf]. | ||
| + | <div style="clear:both"></div> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Urea is highly concentrated in the mammalian kidney to produce the osmotic gradient necessary for water re-absorption. Free diffusion of urea across cell membranes is slow owing to its high polarity, and specialized urea transporters have evolved to achieve rapid and selective urea permeation. Here we present the 2.3 A structure of a functional urea transporter from the bacterium Desulfovibrio vulgaris. The transporter is a homotrimer, and each subunit contains a continuous membrane-spanning pore formed by the two homologous halves of the protein. The pore contains a constricted selectivity filter that can accommodate several dehydrated urea molecules in single file. Backbone and side-chain oxygen atoms provide continuous coordination of urea as it progresses through the filter, and well-placed alpha-helix dipoles provide further compensation for dehydration energy. These results establish that the urea transporter operates by a channel-like mechanism and reveal the physical and chemical basis of urea selectivity. | ||
| - | + | Crystal structure of a bacterial homologue of the kidney urea transporter.,Levin EJ, Quick M, Zhou M Nature. 2009 Dec 10;462(7274):757-61. Epub . PMID:19865084<ref>PMID:19865084</ref> | |
| - | + | ||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
==See Also== | ==See Also== | ||
*[[Urea transporter|Urea transporter]] | *[[Urea transporter|Urea transporter]] | ||
| - | + | == References == | |
| - | == | + | <references/> |
| - | < | + | __TOC__ |
| + | </StructureSection> | ||
[[Category: Desulfovibrio vulgaris]] | [[Category: Desulfovibrio vulgaris]] | ||
| - | [[Category: Levin, E J | + | [[Category: Levin, E J]] |
| - | [[Category: NYCOMPS, New York Consortium on Membrane Protein Structure | + | [[Category: NYCOMPS, New York Consortium on Membrane Protein Structure]] |
| - | [[Category: Zhou, M | + | [[Category: Zhou, M]] |
[[Category: Channel]] | [[Category: Channel]] | ||
[[Category: Membrane protein]] | [[Category: Membrane protein]] | ||
[[Category: New york consortium on membrane protein structure]] | [[Category: New york consortium on membrane protein structure]] | ||
[[Category: Nycomp]] | [[Category: Nycomp]] | ||
| - | [[Category: Protein structure initiative | + | [[Category: PSI, Protein structure initiative]] |
| - | + | ||
[[Category: Structural genomic]] | [[Category: Structural genomic]] | ||
[[Category: Transport protein]] | [[Category: Transport protein]] | ||
[[Category: Transporter]] | [[Category: Transporter]] | ||
[[Category: Urea transport]] | [[Category: Urea transport]] | ||
Revision as of 06:31, 18 December 2014
Crystal Structure of the Urea Transporter from Desulfovibrio Vulgaris
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Categories: Desulfovibrio vulgaris | Levin, E J | NYCOMPS, New York Consortium on Membrane Protein Structure | Zhou, M | Channel | Membrane protein | New york consortium on membrane protein structure | Nycomp | PSI, Protein structure initiative | Structural genomic | Transport protein | Transporter | Urea transport
