3sd3
From Proteopedia
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| - | + | ==The structure of the tetrahydrofolate riboswitch containing a U25C mutation== | |
| - | + | <StructureSection load='3sd3' size='340' side='right' caption='[[3sd3]], [[Resolution|resolution]] 1.95Å' scene=''> | |
| - | { | + | == Structural highlights == |
| + | <table><tr><td colspan='2'>[[3sd3]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3SD3 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3SD3 FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FOZ:N-[4-({[(6S)-2-AMINO-5-FORMYL-4-OXO-1,4,5,6,7,8-HEXAHYDROPTERIDIN-6-YL]METHYL}AMINO)BENZOYL]-L-GLUTAMIC+ACID'>FOZ</scene>, <scene name='pdbligand=IRI:IRIDIUM+HEXAMMINE+ION'>IRI</scene></td></tr> | ||
| + | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3sd1|3sd1]]</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3sd3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3sd3 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3sd3 RCSB], [http://www.ebi.ac.uk/pdbsum/3sd3 PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Transport and biosynthesis of folate and its derivatives are frequently controlled by the tetrahydrofolate (THF) riboswitch in Firmicutes. We have solved the crystal structure of the THF riboswitch aptamer in complex with folinic acid, a THF analog. Uniquely, this structure reveals two molecules of folinic acid binding to a single structured domain. These two sites interact with ligand in a similar fashion, primarily through recognition of the reduced pterin moiety. 7-deazaguanine, a soluble analog of guanine, binds the riboswitch with nearly the same affinity as its natural effector. However, 7-deazaguanine effects transcriptional termination to a substantially lesser degree than folinic acid, suggesting that the cellular guanine pool does not act upon the THF riboswitch. Under physiological conditions the ligands display strong cooperative binding, with one of the two sites playing a greater role in eliciting the regulatory response, which suggests that the second site may play another functional role. | ||
| - | + | The Structure of a Tetrahydrofolate-Sensing Riboswitch Reveals Two Ligand Binding Sites in a Single Aptamer.,Trausch JJ, Ceres P, Reyes FE, Batey RT Structure. 2011 Sep 7. PMID:21906956<ref>PMID:21906956</ref> | |
| - | + | ||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
==See Also== | ==See Also== | ||
*[[Riboswitch|Riboswitch]] | *[[Riboswitch|Riboswitch]] | ||
| - | + | == References == | |
| - | == | + | <references/> |
| - | < | + | __TOC__ |
| - | [[Category: Batey, R T | + | </StructureSection> |
| - | [[Category: Ceres, P | + | [[Category: Batey, R T]] |
| - | [[Category: Reyes, F E | + | [[Category: Ceres, P]] |
| - | [[Category: Trausch, J J | + | [[Category: Reyes, F E]] |
| + | [[Category: Trausch, J J]] | ||
[[Category: Rna]] | [[Category: Rna]] | ||
[[Category: Tetrahydrofolate rna riboswitch]] | [[Category: Tetrahydrofolate rna riboswitch]] | ||
Revision as of 08:28, 18 December 2014
The structure of the tetrahydrofolate riboswitch containing a U25C mutation
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