3pza
From Proteopedia
(Difference between revisions)
| Line 1: | Line 1: | ||
| - | + | ==Fully Reduced (All-ferrous) Pyrococcus rubrerythrin after a 10 second exposure to peroxide.== | |
| - | + | <StructureSection load='3pza' size='340' side='right' caption='[[3pza]], [[Resolution|resolution]] 1.85Å' scene=''> | |
| - | + | == Structural highlights == | |
| + | <table><tr><td colspan='2'>[[3pza]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Pyrococcus_furiosus Pyrococcus furiosus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PZA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3PZA FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FE2:FE+(II)+ION'>FE2</scene>, <scene name='pdbligand=PEO:HYDROGEN+PEROXIDE'>PEO</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3pza FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3pza OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3pza RCSB], [http://www.ebi.ac.uk/pdbsum/3pza PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | High-resolution crystal structures of Pyrococcus furiosus rubrerythrin (PfRbr) in the resting (all-ferrous) state and at time points following exposure of the crystals to hydrogen peroxide are reported. This approach was possible because of the relativity slow turnover of PfRbr at room temperature. To this end, we were able to perform time-dependent peroxide treatment of the fully reduced enzyme, under strictly anaerobic conditions, in the crystalline state. In this work we demonstrate, for the first time, that turnover of a thermophilic rubrerythrin results in approximately 2-A movement of one iron atom in the diiron site from a histidine to a carboxylate ligand. These results confirm that, despite the domain-swapped architecture, the hyperthermophilic rubrerythrins also utilize the classic combination of iron sites together with redox-dependent iron toggling to selectively reduce hydrogen peroxide over dioxygen. In addition, we have identified previously unobserved intermediates in the reaction cycle and observed structural changes that may explain the enzyme precipitation observed for the all-iron form of PfRbr upon oxidation to the all-ferric state. | ||
| - | + | A cryo-crystallographic time course for peroxide reduction by rubrerythrin from Pyrococcus furiosus.,Dillard BD, Demick JM, Adams MW, Lanzilotta WN J Biol Inorg Chem. 2011 Jun 7. PMID:21647777<ref>PMID:21647777</ref> | |
| - | + | ||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
==See Also== | ==See Also== | ||
*[[Journal:JBIC:11|Journal:JBIC:11]] | *[[Journal:JBIC:11|Journal:JBIC:11]] | ||
| - | + | == References == | |
| - | == | + | <references/> |
| - | < | + | __TOC__ |
| + | </StructureSection> | ||
[[Category: Pyrococcus furiosus]] | [[Category: Pyrococcus furiosus]] | ||
| - | [[Category: Adams, M W | + | [[Category: Adams, M W]] |
| - | [[Category: Demick, J M | + | [[Category: Demick, J M]] |
| - | [[Category: Dillard, B D | + | [[Category: Dillard, B D]] |
| - | [[Category: Lanzilotta, W N | + | [[Category: Lanzilotta, W N]] |
[[Category: Oxidoreductase]] | [[Category: Oxidoreductase]] | ||
[[Category: Rubrerythrin]] | [[Category: Rubrerythrin]] | ||
Revision as of 08:44, 18 December 2014
Fully Reduced (All-ferrous) Pyrococcus rubrerythrin after a 10 second exposure to peroxide.
| |||||||||||
