1pj9

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
[[Image:1pj9.jpg|left|200px]]<br /><applet load="1pj9" size="350" color="white" frame="true" align="right" spinBox="true"
+
[[Image:1pj9.jpg|left|200px]]
-
caption="1pj9, resolution 2.00&Aring;" />
+
 
-
'''Bacillus circulans strain 251 loop mutant 183-195'''<br />
+
{{Structure
 +
|PDB= 1pj9 |SIZE=350|CAPTION= <scene name='initialview01'>1pj9</scene>, resolution 2.00&Aring;
 +
|SITE=
 +
|LIGAND= <scene name='pdbligand=MAL:MALTOSE'>MAL</scene>, <scene name='pdbligand=GLC:GLUCOSE'>GLC</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene> and <scene name='pdbligand=ACY:ACETIC ACID'>ACY</scene>
 +
|ACTIVITY= [http://en.wikipedia.org/wiki/Cyclomaltodextrin_glucanotransferase Cyclomaltodextrin glucanotransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.19 2.4.1.19]
 +
|GENE=
 +
}}
 +
 
 +
'''Bacillus circulans strain 251 loop mutant 183-195'''
 +
 
==Overview==
==Overview==
Line 7: Line 16:
==About this Structure==
==About this Structure==
-
1PJ9 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_circulans Bacillus circulans] with <scene name='pdbligand=MAL:'>MAL</scene>, <scene name='pdbligand=GLC:'>GLC</scene>, <scene name='pdbligand=CA:'>CA</scene>, <scene name='pdbligand=MPD:'>MPD</scene> and <scene name='pdbligand=ACY:'>ACY</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Cyclomaltodextrin_glucanotransferase Cyclomaltodextrin glucanotransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.19 2.4.1.19] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PJ9 OCA].
+
1PJ9 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_circulans Bacillus circulans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PJ9 OCA].
==Reference==
==Reference==
-
Improved thermostability of bacillus circulans cyclodextrin glycosyltransferase by the introduction of a salt bridge., Leemhuis H, Rozeboom HJ, Dijkstra BW, Dijkhuizen L, Proteins. 2004 Jan 1;54(1):128-34. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=14705029 14705029]
+
Improved thermostability of bacillus circulans cyclodextrin glycosyltransferase by the introduction of a salt bridge., Leemhuis H, Rozeboom HJ, Dijkstra BW, Dijkhuizen L, Proteins. 2004 Jan 1;54(1):128-34. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/14705029 14705029]
[[Category: Bacillus circulans]]
[[Category: Bacillus circulans]]
[[Category: Cyclomaltodextrin glucanotransferase]]
[[Category: Cyclomaltodextrin glucanotransferase]]
Line 25: Line 34:
[[Category: transferase]]
[[Category: transferase]]
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:29:20 2008''
+
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 13:25:03 2008''

Revision as of 11:25, 20 March 2008


PDB ID 1pj9

Drag the structure with the mouse to rotate
, resolution 2.00Å
Ligands: , , , and
Activity: Cyclomaltodextrin glucanotransferase, with EC number 2.4.1.19
Coordinates: save as pdb, mmCIF, xml



Bacillus circulans strain 251 loop mutant 183-195


Overview

Cyclodextrin glycosyltransferase (CGTase) catalyzes the formation of cyclodextrins from starch. Among the CGTases with known three-dimensional structure, Thermoanaerobacterium thermosulfurigenes CGTase has the highest thermostability. By replacing amino acid residues in the B-domain of Bacillus circulans CGTase with those from T. thermosulfurigenes CGTase, we identified a B. circulans CGTase mutant (with N188D and K192R mutations), with a strongly increased activity half-life at 60 degrees C. Asp188 and Arg192 form a salt bridge in T. thermosulfurigenes CGTase. Structural analysis of the B. circulans CGTase mutant revealed that this salt bridge is also formed in the mutant. Thus, the activity half-life of this enzyme can be enhanced by rational protein engineering.

About this Structure

1PJ9 is a Single protein structure of sequence from Bacillus circulans. Full crystallographic information is available from OCA.

Reference

Improved thermostability of bacillus circulans cyclodextrin glycosyltransferase by the introduction of a salt bridge., Leemhuis H, Rozeboom HJ, Dijkstra BW, Dijkhuizen L, Proteins. 2004 Jan 1;54(1):128-34. PMID:14705029

Page seeded by OCA on Thu Mar 20 13:25:03 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Personal tools