1pjl
From Proteopedia
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| - | [[Image:1pjl.gif|left|200px]] | + | [[Image:1pjl.gif|left|200px]] |
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| - | '''Crystal structure of human m-NAD-ME in ternary complex with NAD and Lu3+''' | + | {{Structure |
| + | |PDB= 1pjl |SIZE=350|CAPTION= <scene name='initialview01'>1pjl</scene>, resolution 2.9Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=LU:LUTETIUM+(III)+ION'>LU</scene> and <scene name='pdbligand=NAD:NICOTINAMIDE-ADENINE-DINUCLEOTIDE'>NAD</scene> | ||
| + | |ACTIVITY= [http://en.wikipedia.org/wiki/Malate_dehydrogenase_(oxaloacetate-decarboxylating) Malate dehydrogenase (oxaloacetate-decarboxylating)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.38 1.1.1.38] | ||
| + | |GENE= ME2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]) | ||
| + | }} | ||
| + | |||
| + | '''Crystal structure of human m-NAD-ME in ternary complex with NAD and Lu3+''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1PJL is a [ | + | 1PJL is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PJL OCA]. |
==Reference== | ==Reference== | ||
| - | Potent and competitive inhibition of malic enzymes by lanthanide ions., Yang Z, Batra R, Floyd DL, Hung HC, Chang GG, Tong L, Biochem Biophys Res Commun. 2000 Aug 2;274(2):440-4. PMID:[http:// | + | Potent and competitive inhibition of malic enzymes by lanthanide ions., Yang Z, Batra R, Floyd DL, Hung HC, Chang GG, Tong L, Biochem Biophys Res Commun. 2000 Aug 2;274(2):440-4. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10913357 10913357] |
[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Malate dehydrogenase (oxaloacetate-decarboxylating)]] | [[Category: Malate dehydrogenase (oxaloacetate-decarboxylating)]] | ||
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[[Category: oxidoreductase]] | [[Category: oxidoreductase]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 13:25:11 2008'' |
Revision as of 11:25, 20 March 2008
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| , resolution 2.9Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | and | ||||||
| Gene: | ME2 (Homo sapiens) | ||||||
| Activity: | Malate dehydrogenase (oxaloacetate-decarboxylating), with EC number 1.1.1.38 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal structure of human m-NAD-ME in ternary complex with NAD and Lu3+
Contents |
Overview
The catalytic activity of malic enzyme (ME), a member of a new class of oxidative decarboxylases, requires the presence of divalent cations (Mn(2+), Mg(2+), and others). The crystal structure at 2.9 A resolution of human mitochondrial NAD(+)-dependent malic enzyme in a ternary complex with NAD(+) and the lanthanide ion Lu(3+), which has similar radius as Mn(2+), reveals a new conformation of the enzyme. The active site in this ternary complex is in an open form, while the organization of the tetramer of the enzyme actually resembles that with a closed active site. The Lu(3+) ion is bound to the enzyme at the same site as Mn(2+). Kinetic studies showed that Lu(3+) is a potent inhibitor of both the human NAD(P)(+)-dependent ME and the NADP(+)-dependent ME from pigeon liver, and is competitive with respect to the divalent cation, consistent with the structural information.
Disease
Known disease associated with this structure: Epilepsy, idopathic generalized, susceptibility to OMIM:[154270]
About this Structure
1PJL is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Potent and competitive inhibition of malic enzymes by lanthanide ions., Yang Z, Batra R, Floyd DL, Hung HC, Chang GG, Tong L, Biochem Biophys Res Commun. 2000 Aug 2;274(2):440-4. PMID:10913357
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