1pk1
From Proteopedia
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| - | [[Image:1pk1.gif|left|200px]] | + | [[Image:1pk1.gif|left|200px]] |
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| - | '''Hetero SAM domain structure of Ph and Scm.''' | + | {{Structure |
| + | |PDB= 1pk1 |SIZE=350|CAPTION= <scene name='initialview01'>1pk1</scene>, resolution 1.80Å | ||
| + | |SITE= | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= | ||
| + | |GENE= PH-P ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=7227 Drosophila melanogaster]), Sex Comb on Midleg ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=7227 Drosophila melanogaster]) | ||
| + | }} | ||
| + | |||
| + | '''Hetero SAM domain structure of Ph and Scm.''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1PK1 is a [ | + | 1PK1 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PK1 OCA]. |
==Reference== | ==Reference== | ||
| - | Structural organization of a Sex-comb-on-midleg/polyhomeotic copolymer., Kim CA, Sawaya MR, Cascio D, Kim W, Bowie JU, J Biol Chem. 2005 Jul 29;280(30):27769-75. Epub 2005 May 19. PMID:[http:// | + | Structural organization of a Sex-comb-on-midleg/polyhomeotic copolymer., Kim CA, Sawaya MR, Cascio D, Kim W, Bowie JU, J Biol Chem. 2005 Jul 29;280(30):27769-75. Epub 2005 May 19. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15905166 15905166] |
[[Category: Drosophila melanogaster]] | [[Category: Drosophila melanogaster]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
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[[Category: Sawaya, M R.]] | [[Category: Sawaya, M R.]] | ||
[[Category: hetero sam domain]] | [[Category: hetero sam domain]] | ||
| - | [[Category: | + | [[Category: polymer]] |
[[Category: transcriptional repression]] | [[Category: transcriptional repression]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 13:25:24 2008'' |
Revision as of 11:25, 20 March 2008
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| , resolution 1.80Å | |||||||
|---|---|---|---|---|---|---|---|
| Gene: | PH-P (Drosophila melanogaster), Sex Comb on Midleg (Drosophila melanogaster) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Hetero SAM domain structure of Ph and Scm.
Overview
The polycomb group proteins are required for the stable maintenance of gene repression patterns established during development. They function as part of large multiprotein complexes created via a multitude of protein-protein interaction domains. Here we examine the interaction between the SAM domains of the polycomb group proteins polyhomeotic (Ph) and Sex-comb-on-midleg (Scm). Previously we showed that Ph-SAM polymerizes as a helical structure. We find that Scm-SAM also polymerizes, and a crystal structure reveals an architecture similar to the Ph-SAM polymer. These results suggest that Ph-SAM and Scm-SAM form a copolymer. Binding affinity measurements between Scm-SAM and Ph-SAM subunits in different orientations indicate a preference for the formation of a single junction copolymer. To provide a model of the copolymer, we determined the structure of the Ph-SAM/Scm-SAM junction. Similar binding modes are observed in both homo- and heterocomplex formation with minimal change in helix axis direction at the polymer joint. The copolymer model suggests that polymeric Scm complexes could extend beyond the local domains of polymeric Ph complexes on chromatin, possibly playing a role in long range repression.
About this Structure
1PK1 is a Protein complex structure of sequences from Drosophila melanogaster. Full crystallographic information is available from OCA.
Reference
Structural organization of a Sex-comb-on-midleg/polyhomeotic copolymer., Kim CA, Sawaya MR, Cascio D, Kim W, Bowie JU, J Biol Chem. 2005 Jul 29;280(30):27769-75. Epub 2005 May 19. PMID:15905166
Page seeded by OCA on Thu Mar 20 13:25:24 2008
