1pkp
From Proteopedia
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| - | [[Image:1pkp.gif|left|200px]] | + | [[Image:1pkp.gif|left|200px]] |
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| - | '''THE STRUCTURE OF RIBOSOMAL PROTEIN S5 REVEALS SITES OF INTERACTION WITH 16S RRNA''' | + | {{Structure |
| + | |PDB= 1pkp |SIZE=350|CAPTION= <scene name='initialview01'>1pkp</scene>, resolution 2.8Å | ||
| + | |SITE= | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= | ||
| + | |GENE= BACILLUS STEAROTHERMOPHILUS ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1422 Geobacillus stearothermophilus]) | ||
| + | }} | ||
| + | |||
| + | '''THE STRUCTURE OF RIBOSOMAL PROTEIN S5 REVEALS SITES OF INTERACTION WITH 16S RRNA''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1PKP is a [ | + | 1PKP is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Geobacillus_stearothermophilus Geobacillus stearothermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PKP OCA]. |
==Reference== | ==Reference== | ||
| - | The structure of ribosomal protein S5 reveals sites of interaction with 16S rRNA., Ramakrishnan V, White SW, Nature. 1992 Aug 27;358(6389):768-71. PMID:[http:// | + | The structure of ribosomal protein S5 reveals sites of interaction with 16S rRNA., Ramakrishnan V, White SW, Nature. 1992 Aug 27;358(6389):768-71. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/1508272 1508272] |
[[Category: Geobacillus stearothermophilus]] | [[Category: Geobacillus stearothermophilus]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: ribosomal protein]] | [[Category: ribosomal protein]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 13:25:36 2008'' |
Revision as of 11:25, 20 March 2008
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| , resolution 2.8Å | |||||||
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| Gene: | BACILLUS STEAROTHERMOPHILUS (Geobacillus stearothermophilus) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
THE STRUCTURE OF RIBOSOMAL PROTEIN S5 REVEALS SITES OF INTERACTION WITH 16S RRNA
Overview
Understanding the process whereby the ribosome translates the genetic code into protein molecules will ultimately require high-resolution structural information, and we report here the first crystal structure of a protein from the small ribosomal subunit. This protein, S5, has a molecular mass of 17,500 and is highly conserved in all lifeforms. The molecule contains two distinct alpha/beta domains that have structural similarities to several other proteins that are components of ribonucleoprotein complexes. Mutations in S5 result in several phenotypes which suggest that S5 may have a role in translational fidelity and translocation. These include ribosome ambiguity or ram, reversion from streptomycin dependence and resistance to spectinomycin. Also, a cold-sensitive, spectinomycin-resistant mutant of S5 has been identified which is defective in initiation. Here we show that these mutations map to two distinct regions of the molecule which seem to be sites of interaction with ribosomal RNA. A structure/function analysis of the molecule reveals discrepancies with current models of the 30S subunit.
About this Structure
1PKP is a Single protein structure of sequence from Geobacillus stearothermophilus. Full crystallographic information is available from OCA.
Reference
The structure of ribosomal protein S5 reveals sites of interaction with 16S rRNA., Ramakrishnan V, White SW, Nature. 1992 Aug 27;358(6389):768-71. PMID:1508272
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