2xga
From Proteopedia
(Difference between revisions)
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| - | + | ==MTSL SPIN-LABELLED SHIGELLA FLEXNERI SPA15== | |
| - | === | + | <StructureSection load='2xga' size='340' side='right' caption='[[2xga]], [[Resolution|resolution]] 2.30Å' scene=''> |
| - | + | == Structural highlights == | |
| + | <table><tr><td colspan='2'>[[2xga]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Shigella_flexneri Shigella flexneri]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XGA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2XGA FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MTN:S-[(1-OXYL-2,2,5,5-TETRAMETHYL-2,5-DIHYDRO-1H-PYRROL-3-YL)METHYL]+METHANESULFONOTHIOATE'>MTN</scene></td></tr> | ||
| + | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1ry9|1ry9]]</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2xga FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xga OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2xga RCSB], [http://www.ebi.ac.uk/pdbsum/2xga PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Shigella flexneri Spa15 is a chaperone of the type 3 secretion system, which binds a number of effectors to ensure their stabilization prior to secretion. One of these effectors is IpgB1, a mimic of the human Ras-like Rho guanosine triphosphatase RhoG. In this study, Spa15 alone and in complex with IpgB1 has been studied by double electron electron resonance, an experiment that gives distance information showing the spacial separation of attached spin labels. This distance is explained by determining the crystal structure of the spin-labeled Spa15 where labels are seen to be buried in hydrophobic pockets. The double electron electron resonance experiment on the Spa15 complex with IpgB1 shows that IpgB1 does not bind Spa15 in the same way as is seen in the homologous Salmonella sp. chaperone:effector complex InvB:SipA. | ||
| - | + | Shigella flexneri Spa15 Crystal Structure Verified in Solution by Double Electron Electron Resonance.,Lillington JE, Lovett JE, Johnson S, Roversi P, Timmel CR, Lea SM J Mol Biol. 2010 Nov 12. PMID:21075116<ref>PMID:21075116</ref> | |
| - | + | ||
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | + | == References == | |
| - | == | + | <references/> |
| - | + | __TOC__ | |
| + | </StructureSection> | ||
[[Category: Shigella flexneri]] | [[Category: Shigella flexneri]] | ||
| - | [[Category: Johnson, S | + | [[Category: Johnson, S]] |
| - | [[Category: Lea, S M | + | [[Category: Lea, S M]] |
| - | [[Category: Lillington, J E.D | + | [[Category: Lillington, J E.D]] |
[[Category: Chaperone]] | [[Category: Chaperone]] | ||
[[Category: Virulence factor]] | [[Category: Virulence factor]] | ||
Revision as of 11:40, 18 December 2014
MTSL SPIN-LABELLED SHIGELLA FLEXNERI SPA15
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