1pkv
From Proteopedia
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| - | [[Image:1pkv.jpg|left|200px]] | + | [[Image:1pkv.jpg|left|200px]] |
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| - | '''The N-terminal domain of riboflavin synthase in complex with riboflavin''' | + | {{Structure |
| + | |PDB= 1pkv |SIZE=350|CAPTION= <scene name='initialview01'>1pkv</scene>, resolution 2.60Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=RBF:RIBOFLAVINE'>RBF</scene> | ||
| + | |ACTIVITY= [http://en.wikipedia.org/wiki/Riboflavin_synthase Riboflavin synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.9 2.5.1.9] | ||
| + | |GENE= RIBE OR RIBC OR B1662 OR SF1690 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli]) | ||
| + | }} | ||
| + | |||
| + | '''The N-terminal domain of riboflavin synthase in complex with riboflavin''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1PKV is a [ | + | 1PKV is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PKV OCA]. |
==Reference== | ==Reference== | ||
| - | The structure of the N-terminal domain of riboflavin synthase in complex with riboflavin at 2.6A resolution., Meining W, Eberhardt S, Bacher A, Ladenstein R, J Mol Biol. 2003 Aug 29;331(5):1053-63. PMID:[http:// | + | The structure of the N-terminal domain of riboflavin synthase in complex with riboflavin at 2.6A resolution., Meining W, Eberhardt S, Bacher A, Ladenstein R, J Mol Biol. 2003 Aug 29;331(5):1053-63. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12927541 12927541] |
[[Category: Escherichia coli]] | [[Category: Escherichia coli]] | ||
[[Category: Riboflavin synthase]] | [[Category: Riboflavin synthase]] | ||
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[[Category: greek key motif]] | [[Category: greek key motif]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 13:25:43 2008'' |
Revision as of 11:25, 20 March 2008
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| , resolution 2.60Å | |||||||
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| Ligands: | |||||||
| Gene: | RIBE OR RIBC OR B1662 OR SF1690 (Escherichia coli) | ||||||
| Activity: | Riboflavin synthase, with EC number 2.5.1.9 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
The N-terminal domain of riboflavin synthase in complex with riboflavin
Overview
Riboflavin synthase of Escherichia coli is a homotrimer with a molecular mass of 70 kDa. The enzyme catalyzes the dismutation of 6,7-dimethyl-8-(1'-D-ribityl)-lumazine, affording riboflavin and 5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione. The N-terminal segment (residues 1-87) and the C-terminal segment (residues 98-187) form beta-barrels with similar fold and a high degree of sequence similarity. A recombinant peptide comprising amino acid residues 1-97 forms a dimer, which binds riboflavin with high affinity. Here, we report the structure of this construct in complex with riboflavin at 2.6A resolution. It is demonstrated that the complex can serve as a model for ligand-binding in the native enzyme. The structure and riboflavin-binding mode is in excellent agreement with structural information obtained from the native enzyme from Escherichia coli and riboflavin synthase from Schizosaccharomyces pombe. The implications for the binding specificity and the regiospecificity of the catalyzed reaction are discussed.
About this Structure
1PKV is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.
Reference
The structure of the N-terminal domain of riboflavin synthase in complex with riboflavin at 2.6A resolution., Meining W, Eberhardt S, Bacher A, Ladenstein R, J Mol Biol. 2003 Aug 29;331(5):1053-63. PMID:12927541
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