2m5b
From Proteopedia
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| - | + | ==The NMR structure of the BID-BAK complex== | |
| - | ===The NMR structure of the BID-BAK complex | + | <StructureSection load='2m5b' size='340' side='right' caption='[[2m5b]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''> |
| - | + | == Structural highlights == | |
| + | <table><tr><td colspan='2'>[[2m5b]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2M5B OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2M5B FirstGlance]. <br> | ||
| + | </td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MK8:2-METHYL-L-NORLEUCINE'>MK8</scene>, <scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene>, <scene name='pdbligand=NLE:NORLEUCINE'>NLE</scene></td></tr> | ||
| + | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2imt|2imt]], [[2bid|2bid]]</td></tr> | ||
| + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">BAK, BAK1, BCL2L7, CDN1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2m5b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2m5b OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2m5b RCSB], [http://www.ebi.ac.uk/pdbsum/2m5b PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The BCL-2-family protein BAK is responsible for mitochondrial outer-membrane permeabilization (MOMP), which leads to apoptosis. The BCL-2 homology 3 (BH3)-only protein BID activates BAK to perform this function. We report the NMR solution structure of the human BID BH3-BAK complex, which identified the activation site at the canonical BH3-binding groove of BAK. Mutating the BAK BH1 in the groove prevented activation and MOMP but not the binding of BID. BAK BH3 mutations allowed BID binding and activation but blunted function by blocking BAK oligomerization. BAK activation follows a 'hit-and-run' mechanism whereby BID dissociates from the trigger site, which allows BAK oligomerization at an overlapping interface. In contrast, the BH3-only proteins NOXA and BAD are predicted to clash with the trigger site and are not activators of BAK. These findings provide insights into the early stages of BAK activation. | ||
| - | + | BID-induced structural changes in BAK promote apoptosis.,Moldoveanu T, Grace CR, Llambi F, Nourse A, Fitzgerald P, Gehring K, Kriwacki RW, Green DR Nat Struct Mol Biol. 2013 May;20(5):589-97. doi: 10.1038/nsmb.2563. Epub 2013 Apr, 21. PMID:23604079<ref>PMID:23604079</ref> | |
| - | + | ||
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | + | == References == | |
| - | == | + | <references/> |
| - | + | __TOC__ | |
| + | </StructureSection> | ||
[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
| - | [[Category: Grace, C R | + | [[Category: Grace, C R]] |
| - | [[Category: Green, D R | + | [[Category: Green, D R]] |
| - | [[Category: Kriwacki, R W | + | [[Category: Kriwacki, R W]] |
| - | [[Category: Moldoveanu, T | + | [[Category: Moldoveanu, T]] |
[[Category: Apoptosis]] | [[Category: Apoptosis]] | ||
[[Category: Bcl-2 family effector bak]] | [[Category: Bcl-2 family effector bak]] | ||
Revision as of 11:46, 18 December 2014
The NMR structure of the BID-BAK complex
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