2k1i
From Proteopedia
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| - | + | ==Synthesis, Structure and Activities of an Oral Mucosal Alpha-Defensin from Rhesus Macaque== | |
| - | + | <StructureSection load='2k1i' size='340' side='right' caption='[[2k1i]], [[NMR_Ensembles_of_Models | 10 NMR models]]' scene=''> | |
| - | + | == Structural highlights == | |
| + | <table><tr><td colspan='2'>[[2k1i]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2K1I OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2K1I FirstGlance]. <br> | ||
| + | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2k1i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2k1i OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2k1i RCSB], [http://www.ebi.ac.uk/pdbsum/2k1i PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The oral cavity is an environment challenged by a large variety of pathogens. Consequently, the antimicrobial peptides expressed in that environment are interesting as they evolved to defend against a broad spectrum of bacteria and fungi. Here we report the discovery of new alpha-defensins from rhesus macaque oral mucosa and determine the first alpha-defensin structure from that species. The new peptides were identified by sequencing of reverse transcriptase-PCR products obtained from oral mucosal tissues, disclosing three mucosal alpha-defensins, termed rhesus macaque oral alpha-defensins (ROADs). The peptide corresponding to fully processed ROAD-1 was synthesized, subjected to folding/oxidation conditions, and purified. ROAD-1 was active against Staphylococcus aureus, Escherichia coli, and Candida albicans in a concentration-dependent manner. We determined the structure of ROAD-1 using NMR spectroscopy and find that the synthetic peptide adopts the canonical disulfide pairing and alpha-defensin fold. The antimicrobial mechanism of defensins has been correlated with their ability to disrupt and permeabilize the cell envelope, activities that depend on the surface features of the folded peptide. Although ROAD-1 maintains the defensin fold, the oral defensin displays distinct surface features when compared with other alpha-defensin structures. | ||
| - | + | Synthesis, structure, and activities of an oral mucosal alpha-defensin from rhesus macaque.,Vasudevan S, Yuan J, Osapay G, Tran P, Tai K, Liang W, Kumar V, Selsted ME, Cocco MJ J Biol Chem. 2008 Dec 19;283(51):35869-77. Epub 2008 Oct 17. PMID:18930922<ref>PMID:18930922</ref> | |
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| - | == | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> |
| - | + | </div> | |
| - | [[Category: Cocco, M J | + | |
| - | [[Category: Osapay, G | + | ==See Also== |
| - | [[Category: Selsted, M | + | *[[Defensin|Defensin]] |
| - | [[Category: Tai, K | + | == References == |
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Cocco, M J]] | ||
| + | [[Category: Osapay, G]] | ||
| + | [[Category: Selsted, M]] | ||
| + | [[Category: Tai, K]] | ||
[[Category: Tran,P]] | [[Category: Tran,P]] | ||
| - | [[Category: Vasudevan, S V | + | [[Category: Vasudevan, S V]] |
| - | [[Category: Yuan, J | + | [[Category: Yuan, J]] |
[[Category: Anti parallel beta sheet]] | [[Category: Anti parallel beta sheet]] | ||
[[Category: Antimicrobial peptide]] | [[Category: Antimicrobial peptide]] | ||
Revision as of 11:54, 18 December 2014
Synthesis, Structure and Activities of an Oral Mucosal Alpha-Defensin from Rhesus Macaque
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