2ltq

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{{Large structure}}
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==High resolution structure of DsbB C41S by joint calculation with solid-state NMR and X-ray data==
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{{STRUCTURE_2ltq| PDB=2ltq | SCENE= }}
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<StructureSection load='2ltq' size='340' side='right' caption='[[2ltq]], [[NMR_Ensembles_of_Models | 10 NMR models]]' scene=''>
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===High resolution structure of DsbB C41S by joint calculation with solid-state NMR and X-ray data===
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== Structural highlights ==
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{{ABSTRACT_PUBMED_23416557}}
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<table><tr><td colspan='2'>[[2ltq]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli_k-12 Escherichia coli k-12] and [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LTQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2LTQ FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=UQ1:UBIQUINONE-1'>UQ1</scene></td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2leg|2leg]], [[2zuq|2zuq]]</td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">b1185, dsbB, JW5182, roxB, ycgA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 Escherichia coli K-12])</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ltq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ltq OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2ltq RCSB], [http://www.ebi.ac.uk/pdbsum/2ltq PDBsum]</span></td></tr>
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</table>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The integral membrane protein DsbB in Escherichia coli is responsible for oxidizing the periplasmic protein DsbA, which forms disulfide bonds in substrate proteins. We have developed a high-resolution structural model by combining experimental X-ray and solid-state NMR with molecular dynamics (MD) simulations. We embedded the high-resolution DsbB structure, derived from the joint calculation with X-ray reflections and solid-state NMR restraints, into the lipid bilayer and performed MD simulations to provide a mechanistic view of DsbB function in the membrane. Further, we revealed the membrane topology of DsbB by selective proton spin diffusion experiments, which directly probe the correlations of DsbB with water and lipid acyl chains. NMR data also support the model of a flexible periplasmic loop and an interhelical hydrogen bond between Glu26 and Tyr153.
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==About this Structure==
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Structure of the Disulfide Bond Generating Membrane Protein DsbB in the Lipid Bilayer.,Tang M, Nesbitt AE, Sperling LJ, Berthold DA, Schwieters CD, Gennis RB, Rienstra CM J Mol Biol. 2013 Feb 14. pii: S0022-2836(13)00095-8. doi:, 10.1016/j.jmb.2013.02.009. PMID:23416557<ref>PMID:23416557</ref>
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[[2ltq]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli_k-12 Escherichia coli k-12] and [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LTQ OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<ref group="xtra">PMID:023416557</ref><references group="xtra"/><references/>
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</div>
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==See Also==
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*[[Protein disulfide oxidoreductase|Protein disulfide oxidoreductase]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
[[Category: Escherichia coli k-12]]
[[Category: Escherichia coli k-12]]
[[Category: Mus musculus]]
[[Category: Mus musculus]]
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[[Category: Gennis, R B.]]
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[[Category: Gennis, R B]]
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[[Category: Nesbitt, A E.]]
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[[Category: Nesbitt, A E]]
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[[Category: Rienstra, C M.]]
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[[Category: Rienstra, C M]]
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[[Category: Schwieters, C D.]]
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[[Category: Schwieters, C D]]
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[[Category: Sperling, L J.]]
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[[Category: Sperling, L J]]
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[[Category: Tang, M.]]
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[[Category: Tang, M]]
[[Category: Cell inner membrane]]
[[Category: Cell inner membrane]]
[[Category: Cell membrane]]
[[Category: Cell membrane]]

Revision as of 12:14, 18 December 2014

High resolution structure of DsbB C41S by joint calculation with solid-state NMR and X-ray data

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