1pqt

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[[Image:1pqt.gif|left|200px]]<br /><applet load="1pqt" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:1pqt.gif|left|200px]]
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caption="1pqt" />
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'''REFINEMENT OF d(GCGAAGC) HAIRPIN STRUCTURE USING ONE- AND TWO-BOND RESIDUAL DIPOLAR COUPLINGS'''<br />
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{{Structure
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|PDB= 1pqt |SIZE=350|CAPTION= <scene name='initialview01'>1pqt</scene>
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|SITE=
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|LIGAND=
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|ACTIVITY=
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|GENE=
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}}
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'''REFINEMENT OF d(GCGAAGC) HAIRPIN STRUCTURE USING ONE- AND TWO-BOND RESIDUAL DIPOLAR COUPLINGS'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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1PQT is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PQT OCA].
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1PQT is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PQT OCA].
==Reference==
==Reference==
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Refinement of d(GCGAAGC) hairpin structure using one- and two-bond residual dipolar couplings., Padrta P, Stefl R, Kralik L, Zidek L, Sklenar V, J Biomol NMR. 2002 Sep;24(1):1-14. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12449414 12449414]
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Refinement of d(GCGAAGC) hairpin structure using one- and two-bond residual dipolar couplings., Padrta P, Stefl R, Kralik L, Zidek L, Sklenar V, J Biomol NMR. 2002 Sep;24(1):1-14. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12449414 12449414]
[[Category: Protein complex]]
[[Category: Protein complex]]
[[Category: Padrta, P.]]
[[Category: Padrta, P.]]
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[[Category: hairpin]]
[[Category: hairpin]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:31:36 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 13:27:48 2008''

Revision as of 11:27, 20 March 2008

Image:1pqt.gif


PDB ID 1pqt

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Coordinates: save as pdb, mmCIF, xml



REFINEMENT OF d(GCGAAGC) HAIRPIN STRUCTURE USING ONE- AND TWO-BOND RESIDUAL DIPOLAR COUPLINGS


Overview

The structure of the 13C,15N-labeled d(GCGAAGC) hairpin, as determined by NMR spectroscopy and refined using molecular dynamics with NOE-derived distances, torsion angles, and residual dipolar couplings (RDCs), is presented. Although the studied molecule is of small size, it is demonstrated that the incorporation of diminutive RDCs can significantly improve local structure determination of regions undefined by the conventional restraints. Very good correlation between the experimental and back-calculated small one- and two-bond 1H-13C, 1H-15N, 13C-13C and 13C-15N coupling constants has been attained. The final structures clearly show typical features of the miniloop architecture. The structure is discussed in context of the extraordinary stability of the d(GCGAAGC) hairpin, which originates from a complex interplay between the aromatic base stacking and hydrogen bonding interactions.

About this Structure

1PQT is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.

Reference

Refinement of d(GCGAAGC) hairpin structure using one- and two-bond residual dipolar couplings., Padrta P, Stefl R, Kralik L, Zidek L, Sklenar V, J Biomol NMR. 2002 Sep;24(1):1-14. PMID:12449414

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