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2xb6

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{{STRUCTURE_2xb6| PDB=2xb6 | SCENE= }}
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==REVISITED CRYSTAL STRUCTURE OF NEUREXIN1BETA-NEUROLIGIN4 COMPLEX==
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===REVISITED CRYSTAL STRUCTURE OF NEUREXIN1BETA-NEUROLIGIN4 COMPLEX===
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<StructureSection load='2xb6' size='340' side='right' caption='[[2xb6]], [[Resolution|resolution]] 2.60&Aring;' scene=''>
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{{ABSTRACT_PUBMED_20543817}}
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2xb6]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XB6 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2XB6 FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MES:2-(N-MORPHOLINO)-ETHANESULFONIC+ACID'>MES</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1c4r|1c4r]], [[2vh8|2vh8]], [[2wqz|2wqz]]</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2xb6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xb6 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2xb6 RCSB], [http://www.ebi.ac.uk/pdbsum/2xb6 PDBsum]</span></td></tr>
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</table>
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== Disease ==
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[[http://www.uniprot.org/uniprot/NLGNX_HUMAN NLGNX_HUMAN]] Defects in NLGN4X may be the cause of susceptibility to autism X-linked type 2 (AUTSX2) [MIM:[http://omim.org/entry/300495 300495]]. AUTSX2 is a pervasive developmental disorder (PDD), prototypically characterized by impairments in reciprocal social interaction and communication, restricted and stereotyped patterns of interests and activities, and the presence of developmental abnormalities by 3 years of age.<ref>PMID:12669065</ref> Defects in NLGN4X may be the cause of susceptibility to X-linked Asperger syndrome 2 (ASPGX2) [MIM:[http://omim.org/entry/300497 300497]]. ASPGX2 is considered to be a form of childhood autism.
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== Function ==
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[[http://www.uniprot.org/uniprot/NLGNX_HUMAN NLGNX_HUMAN]] Putative neuronal cell surface protein involved in cell-cell-interactions. [[http://www.uniprot.org/uniprot/NRX1B_RAT NRX1B_RAT]] Neuronal cell surface protein that may be involved in cell recognition and cell adhesion by forming intracellular junctions through binding to neuroligins. May play a role in formation or maintenance of synaptic junctions. May mediate intracellular signaling. May play a role in angiogenesis (By similarity).<ref>PMID:9325340</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/xb/2xb6_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The extracellular domains of neuroligins and neurexins interact through Ca(2+) to form flexible trans-synaptic associations characterized by selectivity for neuroligin or neurexin subtypes. This heterophilic interaction, essential for synaptic maturation and differentiation, is regulated by gene selection, alternative mRNA splicing and post-translational modifications. A new, 2.6 A-resolution crystal structure of a soluble neurexin-1beta-neuroligin-4 (Nrx1beta-NL4) complex permits a detailed description of the Ca(2+)-coordinated interface and unveils concerted positional rearrangements of several residues of NL4, not observed in neuroligin-1, associated with Nrx1beta binding. Surface plasmon resonance analysis of the binding of structure-guided Nrx1beta mutants towards NL4 and neuroligin-1 shows that flexibility of the Nrx1beta-binding site in NL4 is reflected in a greater dissociation constant of the complex and higher sensitivity to ionic strength and pH variations. Analysis of neuroligin mutants points to critical functions for two respective residues in neuroligin-1 and neuroligin-2 in governing the affinity of the complexes. Although neuroligin-1 and neuroligin-2 have pre-determined conformations that respectively promote and prevent Nrx1beta association, unique conformational reshaping of the NL4 surface is required to permit Nrx1beta association.
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==Disease==
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Structural insights into the exquisite selectivity of neurexin/neuroligin synaptic interactions.,Leone P, Comoletti D, Ferracci G, Conrod S, Garcia SU, Taylor P, Bourne Y, Marchot P EMBO J. 2010 Jul 21;29(14):2461-71. Epub 2010 Jun 11. PMID:20543817<ref>PMID:20543817</ref>
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[[http://www.uniprot.org/uniprot/NLGNX_HUMAN NLGNX_HUMAN]] Defects in NLGN4X may be the cause of susceptibility to autism X-linked type 2 (AUTSX2) [MIM:[http://omim.org/entry/300495 300495]]. AUTSX2 is a pervasive developmental disorder (PDD), prototypically characterized by impairments in reciprocal social interaction and communication, restricted and stereotyped patterns of interests and activities, and the presence of developmental abnormalities by 3 years of age.<ref>PMID:12669065</ref> Defects in NLGN4X may be the cause of susceptibility to X-linked Asperger syndrome 2 (ASPGX2) [MIM:[http://omim.org/entry/300497 300497]]. ASPGX2 is considered to be a form of childhood autism.
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==Function==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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[[http://www.uniprot.org/uniprot/NLGNX_HUMAN NLGNX_HUMAN]] Putative neuronal cell surface protein involved in cell-cell-interactions. [[http://www.uniprot.org/uniprot/NRX1B_RAT NRX1B_RAT]] Neuronal cell surface protein that may be involved in cell recognition and cell adhesion by forming intracellular junctions through binding to neuroligins. May play a role in formation or maintenance of synaptic junctions. May mediate intracellular signaling. May play a role in angiogenesis (By similarity).<ref>PMID:9325340</ref>
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</div>
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==About this Structure==
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[[2xb6]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XB6 OCA].
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==See Also==
==See Also==
*[[Neurexin|Neurexin]]
*[[Neurexin|Neurexin]]
*[[Neuroligin|Neuroligin]]
*[[Neuroligin|Neuroligin]]
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== References ==
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==Reference==
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<references/>
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<ref group="xtra">PMID:020543817</ref><references group="xtra"/><references/>
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__TOC__
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</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
[[Category: Rattus norvegicus]]
[[Category: Rattus norvegicus]]
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[[Category: Bourne, Y.]]
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[[Category: Bourne, Y]]
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[[Category: Comoletti, D.]]
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[[Category: Comoletti, D]]
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[[Category: Conrod, S.]]
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[[Category: Conrod, S]]
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[[Category: Ferracci, G.]]
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[[Category: Ferracci, G]]
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[[Category: Garcia, S U.]]
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[[Category: Garcia, S U]]
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[[Category: Leone, P.]]
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[[Category: Leone, P]]
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[[Category: Marchot, P.]]
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[[Category: Marchot, P]]
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[[Category: Taylor, P.]]
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[[Category: Taylor, P]]
[[Category: Alpha-beta-hydrolase fold]]
[[Category: Alpha-beta-hydrolase fold]]
[[Category: Autism]]
[[Category: Autism]]
[[Category: Cell adhesion]]
[[Category: Cell adhesion]]
[[Category: Conformational rearrangement]]
[[Category: Conformational rearrangement]]

Revision as of 12:22, 18 December 2014

REVISITED CRYSTAL STRUCTURE OF NEUREXIN1BETA-NEUROLIGIN4 COMPLEX

2xb6, resolution 2.60Å

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