2xed
From Proteopedia
(Difference between revisions)
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| - | + | ==NOCARDIA FARCINICA MALEATE CIS-TRANS ISOMERASE C194S MUTANT WITH A COVALENTLY BOUND SUCCINYLCYSTEINE INTERMEDIATE== | |
| - | + | <StructureSection load='2xed' size='340' side='right' caption='[[2xed]], [[Resolution|resolution]] 1.95Å' scene=''> | |
| - | + | == Structural highlights == | |
| + | <table><tr><td colspan='2'>[[2xed]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Nocardia_farcinica Nocardia farcinica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XED OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2XED FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SIN:SUCCINIC+ACID'>SIN</scene></td></tr> | ||
| + | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2xec|2xec]]</td></tr> | ||
| + | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Maleate_isomerase Maleate isomerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.2.1.1 5.2.1.1] </span></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2xed FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xed OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2xed RCSB], [http://www.ebi.ac.uk/pdbsum/2xed PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | == Evolutionary Conservation == | ||
| + | [[Image:Consurf_key_small.gif|200px|right]] | ||
| + | Check<jmol> | ||
| + | <jmolCheckbox> | ||
| + | <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/xe/2xed_consurf.spt"</scriptWhenChecked> | ||
| + | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | ||
| + | <text>to colour the structure by Evolutionary Conservation</text> | ||
| + | </jmolCheckbox> | ||
| + | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf]. | ||
| + | <div style="clear:both"></div> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Maleate isomerase (MI), a member of the Asp/Glu racemase superfamily, catalyzes cis-trans isomerization of the C2-C3 double bond in maleate to yield fumarate. Mutational studies, in conjunction with the structure of the C194A mutant of Nocardia farcinica MI cocrystallized with maleate, have revealed an unprecedented mode of catalysis for the superfamily in which the isomerization reaction is initiated by nucleophilic attack of cysteine at the double bond, yielding a covalent succinylcysteine-like intermediate. | ||
| - | + | A Covalent Succinylcysteine-like Intermediate in the Enzyme-Catalyzed Transformation of Maleate to Fumarate by Maleate Isomerase.,Fisch F, Fleites CM, Delenne M, Baudendistel N, Hauer B, Turkenburg JP, Hart S, Bruce NC, Grogan G J Am Chem Soc. 2010 Aug 2. PMID:20677745<ref>PMID:20677745</ref> | |
| - | + | ||
| - | == | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> |
| - | + | </div> | |
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
[[Category: Maleate isomerase]] | [[Category: Maleate isomerase]] | ||
[[Category: Nocardia farcinica]] | [[Category: Nocardia farcinica]] | ||
| - | [[Category: Baudendistel, N | + | [[Category: Baudendistel, N]] |
| - | [[Category: Bruce, N C | + | [[Category: Bruce, N C]] |
| - | [[Category: Fisch, F | + | [[Category: Fisch, F]] |
| - | [[Category: Grogan, G | + | [[Category: Grogan, G]] |
| - | [[Category: Hart, S | + | [[Category: Hart, S]] |
| - | [[Category: Hauer, B | + | [[Category: Hauer, B]] |
| - | [[Category: Martinez-Fleites, C | + | [[Category: Martinez-Fleites, C]] |
| - | [[Category: Turkenburg, J P | + | [[Category: Turkenburg, J P]] |
[[Category: Cofactor-independent cis-trans isomerase]] | [[Category: Cofactor-independent cis-trans isomerase]] | ||
[[Category: Isomerase]] | [[Category: Isomerase]] | ||
[[Category: Nicotinic acid catabolism]] | [[Category: Nicotinic acid catabolism]] | ||
Revision as of 12:23, 18 December 2014
NOCARDIA FARCINICA MALEATE CIS-TRANS ISOMERASE C194S MUTANT WITH A COVALENTLY BOUND SUCCINYLCYSTEINE INTERMEDIATE
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