This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


2lq8

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
{{STRUCTURE_2lq8| PDB=2lq8 | SCENE= }}
+
==Domain interaction in Thermotoga maritima NusG==
-
===Domain interaction in Thermotoga maritima NusG===
+
<StructureSection load='2lq8' size='340' side='right' caption='[[2lq8]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''>
-
{{ABSTRACT_PUBMED_23415559}}
+
== Structural highlights ==
 +
<table><tr><td colspan='2'>[[2lq8]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Thermotoga_maritima Thermotoga maritima]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LQ8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2LQ8 FirstGlance]. <br>
 +
</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">nusG, TM_0453 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2336 Thermotoga maritima])</td></tr>
 +
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2lq8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2lq8 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2lq8 RCSB], [http://www.ebi.ac.uk/pdbsum/2lq8 PDBsum]</span></td></tr>
 +
</table>
 +
<div style="background-color:#fffaf0;">
 +
== Publication Abstract from PubMed ==
 +
NusG is a conserved regulatory protein interacting with RNA polymerase (RNAP) and other proteins to form multicomponent complexes that modulate transcription. The crystal structure of Thermotoga maritima NusG (TmNusG) shows a three-domain architecture, comprising well-conserved amino-terminal (NTD) and carboxy-terminal (CTD) domains with an additional, species-specific domain inserted into the NTD. NTD and CTD directly contact each other, occluding a surface of the NTD for binding to RNAP and a surface on the CTD interacting either with transcription termination factor Rho or transcription antitermination factor NusE. NMR spectroscopy confirmed the intramolecular NTD-CTD interaction up to the optimal growth temperature of Thermotoga maritima. The domain interaction involves a dynamic equilibrium between open and closed states and contributes significantly to the overall fold stability of the protein. Wild-type TmNusG and deletion variants could not replace endogenous Escherichia coli NusG, suggesting that the NTD-CTD interaction of TmNusG represents an autoinhibited state.
-
==Function==
+
An Autoinhibited State in the Structure of Thermotoga maritima NusG.,Drogemuller J, Stegmann CM, Mandal A, Steiner T, Burmann BM, Gottesman ME, Wohrl BM, Rosch P, Wahl MC, Schweimer K Structure. 2013 Feb 12. pii: S0969-2126(13)00008-7. doi:, 10.1016/j.str.2012.12.015. PMID:23415559<ref>PMID:23415559</ref>
-
[[http://www.uniprot.org/uniprot/NUSG_THEMA NUSG_THEMA]] Influences transcription termination and antitermination. Acts as a component of the transcription complex, and interacts with the termination factor rho and RNA polymerase (By similarity).
+
-
==About this Structure==
+
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-
[[2lq8]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Thermotoga_maritima Thermotoga maritima]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LQ8 OCA].
+
</div>
 +
== References ==
 +
<references/>
 +
__TOC__
 +
</StructureSection>
[[Category: Thermotoga maritima]]
[[Category: Thermotoga maritima]]
-
[[Category: Burmann, B.]]
+
[[Category: Burmann, B]]
-
[[Category: Droegemueller, J.]]
+
[[Category: Droegemueller, J]]
-
[[Category: Roesch, P.]]
+
[[Category: Roesch, P]]
-
[[Category: Schweimer, K.]]
+
[[Category: Schweimer, K]]
-
[[Category: Stegmann, C.]]
+
[[Category: Stegmann, C]]
-
[[Category: Wahl, M C.]]
+
[[Category: Wahl, M C]]
[[Category: Transcription]]
[[Category: Transcription]]

Revision as of 12:24, 18 December 2014

Domain interaction in Thermotoga maritima NusG

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2lq8"
Personal tools