1pu7
From Proteopedia
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| - | [[Image:1pu7.gif|left|200px]] | + | [[Image:1pu7.gif|left|200px]] |
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| - | '''Crystal structure of H.pylori 3-methyladenine DNA glycosylase (MagIII) bound to 3,9-dimethyladenine''' | + | {{Structure |
| + | |PDB= 1pu7 |SIZE=350|CAPTION= <scene name='initialview01'>1pu7</scene>, resolution 1.93Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=39A:6-AMINO-3,9-DIMETHYL-9H-PURIN-3-IUM'>39A</scene> and <scene name='pdbligand=BME:BETA-MERCAPTOETHANOL'>BME</scene> | ||
| + | |ACTIVITY= | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''Crystal structure of H.pylori 3-methyladenine DNA glycosylase (MagIII) bound to 3,9-dimethyladenine''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1PU7 is a [ | + | 1PU7 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Helicobacter_pylori Helicobacter pylori]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PU7 OCA]. |
==Reference== | ==Reference== | ||
| - | Crystal structures of 3-methyladenine DNA glycosylase MagIII and the recognition of alkylated bases., Eichman BF, O'Rourke EJ, Radicella JP, Ellenberger T, EMBO J. 2003 Oct 1;22(19):4898-909. PMID:[http:// | + | Crystal structures of 3-methyladenine DNA glycosylase MagIII and the recognition of alkylated bases., Eichman BF, O'Rourke EJ, Radicella JP, Ellenberger T, EMBO J. 2003 Oct 1;22(19):4898-909. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/14517230 14517230] |
[[Category: Helicobacter pylori]] | [[Category: Helicobacter pylori]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: helix-hairpin-helix]] | [[Category: helix-hairpin-helix]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 13:29:00 2008'' |
Revision as of 11:29, 20 March 2008
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| , resolution 1.93Å | |||||||
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| Ligands: | and | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal structure of H.pylori 3-methyladenine DNA glycosylase (MagIII) bound to 3,9-dimethyladenine
Overview
DNA glycosylases catalyze the excision of chemically modified bases from DNA. Although most glycosylases are specific to a particular base, the 3-methyladenine (m3A) DNA glycosylases include both highly specific enzymes acting on a single modified base, and enzymes with broader specificity for alkylation-damaged DNA. Our structural understanding of these different enzymatic specificities is currently limited to crystal and NMR structures of the unliganded enzymes and complexes with abasic DNA inhibitors. Presented here are high-resolution crystal structures of the m3A DNA glycosylase from Helicobacter pylori (MagIII) in the unliganded form and bound to alkylated bases 3,9-dimethyladenine and 1,N6-ethenoadenine. These are the first structures of a nucleobase bound in the active site of a m3A glycosylase belonging to the helix-hairpin-helix superfamily. MagIII achieves its specificity for positively-charged m3A not by direct interactions with purine or methyl substituent atoms, but rather by stacking the base between two aromatic side chains in a pocket that excludes 7-methylguanine. We report base excision and DNA binding activities of MagIII active site mutants, together with a structural comparison of the HhH glycosylases.
About this Structure
1PU7 is a Single protein structure of sequence from Helicobacter pylori. Full crystallographic information is available from OCA.
Reference
Crystal structures of 3-methyladenine DNA glycosylase MagIII and the recognition of alkylated bases., Eichman BF, O'Rourke EJ, Radicella JP, Ellenberger T, EMBO J. 2003 Oct 1;22(19):4898-909. PMID:14517230
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