1pfi

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{{STRUCTURE_1pfi| PDB=1pfi | SCENE= }}
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==PF1 VIRUS STRUCTURE: HELICAL COAT PROTEIN AND DNA WITH PARAXIAL PHOSPHATES==
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===PF1 VIRUS STRUCTURE: HELICAL COAT PROTEIN AND DNA WITH PARAXIAL PHOSPHATES===
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<StructureSection load='1pfi' size='340' side='right' caption='[[1pfi]], [[Resolution|resolution]] 3.00&Aring;' scene=''>
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{{ABSTRACT_PUBMED_8036516}}
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1pfi]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseudomonas_phage_pf1 Pseudomonas phage pf1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PFI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1PFI FirstGlance]. <br>
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</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1pfi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1pfi OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1pfi RCSB], [http://www.ebi.ac.uk/pdbsum/1pfi PDBsum]</span></td></tr>
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</table>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The helical path of the DNA in filamentous bacteriophage Pf1 was deduced from different kinds of existing structural information, including results from x-ray fiber diffraction. The DNA has the same pitch, 16 angstroms, as the surrounding helix of protein subunits; the rise and rotation per nucleotides are 6.1 angstroms and 132 degrees, respectively; and the phosphates are 2.5 angstroms from the axis. The DNA in Pf1 is, therefore, the most extended and twisted DNA structure known. On the basis of the DNA structure and extensive additional information about the protein, a model of the virion is proposed. In the model, the DNA bases reach out, into the protein, and the lysine and arginine side chains reach in, between the DNA bases, to stabilize the paraxial phosphate charges; the conformation of the protein subunit is a combination of alpha and 3(10) helices.
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==Function==
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Pf1 virus structure: helical coat protein and DNA with paraxial phosphates.,Liu DJ, Day LA Science. 1994 Jul 29;265(5172):671-4. PMID:8036516<ref>PMID:8036516</ref>
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[[http://www.uniprot.org/uniprot/CAPSD_BPPF1 CAPSD_BPPF1]] Self assembles to form a helical capsid wrapping up the viral genomic DNA. The capsid displays a filamentous structure with a length of 760-1950 nm and a width of 6-8 nm. The virion assembly and budding take place at the host inner membrane (By similarity).
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==About this Structure==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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[[1pfi]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseudomonas_phage_pf1 Pseudomonas phage pf1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PFI OCA].
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</div>
==See Also==
==See Also==
*[[Virus coat protein|Virus coat protein]]
*[[Virus coat protein|Virus coat protein]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
[[Category: Pseudomonas phage pf1]]
[[Category: Pseudomonas phage pf1]]
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[[Category: Day, L A.]]
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[[Category: Day, L A]]
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[[Category: Liu, D J.]]
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[[Category: Liu, D J]]
[[Category: Helical virus]]
[[Category: Helical virus]]
[[Category: Virus]]
[[Category: Virus]]

Revision as of 12:51, 18 December 2014

PF1 VIRUS STRUCTURE: HELICAL COAT PROTEIN AND DNA WITH PARAXIAL PHOSPHATES

1pfi, resolution 3.00Å

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