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1pvs
From Proteopedia
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| - | [[Image:1pvs.gif|left|200px]] | + | [[Image:1pvs.gif|left|200px]] |
| - | + | ||
| - | '''3-methyladenine Glcosylase II(AlkA) Hypoxanthine complex''' | + | {{Structure |
| + | |PDB= 1pvs |SIZE=350|CAPTION= <scene name='initialview01'>1pvs</scene>, resolution 2.40Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=7HP:7-HYDROXY-PYRAZOLO[4,3-D]PYRIMIDINE'>7HP</scene> | ||
| + | |ACTIVITY= [http://en.wikipedia.org/wiki/DNA-3-methyladenine_glycosylase_II DNA-3-methyladenine glycosylase II], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.2.21 3.2.2.21] | ||
| + | |GENE= ALKA OR AIDA OR B2068 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli]) | ||
| + | }} | ||
| + | |||
| + | '''3-methyladenine Glcosylase II(AlkA) Hypoxanthine complex''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1PVS is a [ | + | 1PVS is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PVS OCA]. |
==Reference== | ==Reference== | ||
| - | 3-methyladenine-DNA glycosylase II: the crystal structure of an AlkA-hypoxanthine complex suggests the possibility of product inhibition., Teale M, Symersky J, DeLucas L, Bioconjug Chem. 2002 May-Jun;13(3):403-7. PMID:[http:// | + | 3-methyladenine-DNA glycosylase II: the crystal structure of an AlkA-hypoxanthine complex suggests the possibility of product inhibition., Teale M, Symersky J, DeLucas L, Bioconjug Chem. 2002 May-Jun;13(3):403-7. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12009927 12009927] |
[[Category: DNA-3-methyladenine glycosylase II]] | [[Category: DNA-3-methyladenine glycosylase II]] | ||
[[Category: Escherichia coli]] | [[Category: Escherichia coli]] | ||
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[[Category: reaction-product complex]] | [[Category: reaction-product complex]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 13:29:35 2008'' |
Revision as of 11:29, 20 March 2008
| |||||||
| , resolution 2.40Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | |||||||
| Gene: | ALKA OR AIDA OR B2068 (Escherichia coli) | ||||||
| Activity: | DNA-3-methyladenine glycosylase II, with EC number 3.2.2.21 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
3-methyladenine Glcosylase II(AlkA) Hypoxanthine complex
Overview
Escherichia coli (E. coli) protein 3-methyladenine-DNA glycosylase II (AlkA) functions primarily by removing alkylation damage from duplex and single stranded DNA. A crystal structure of AlkA was refined to 2.0 A resolution. This structure in turn was used to refine an AlkA-hypoxanthine (substrate) complex structure to 2.4 A resolution. The complex structure shows hypoxanthine located in AlkA's active site stacked between residues W218 and Y239. The structural analysis of the AlkA and AlkA-hypoxanthine structures indicate that free hypoxanthine binding in the active site may inhibit glycosylase activity.
About this Structure
1PVS is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.
Reference
3-methyladenine-DNA glycosylase II: the crystal structure of an AlkA-hypoxanthine complex suggests the possibility of product inhibition., Teale M, Symersky J, DeLucas L, Bioconjug Chem. 2002 May-Jun;13(3):403-7. PMID:12009927
Page seeded by OCA on Thu Mar 20 13:29:35 2008
