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3b29

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{{STRUCTURE_3b29| PDB=3b29 | SCENE= }}
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==Human leukotriene C4 synthase in complex with dodecyl-beta-D-selenomaltoside==
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===Human leukotriene C4 synthase in complex with dodecyl-beta-D-selenomaltoside===
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<StructureSection load='3b29' size='340' side='right' caption='[[3b29]], [[Resolution|resolution]] 3.20&Aring;' scene=''>
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{{ABSTRACT_PUBMED_22139193}}
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== Structural highlights ==
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<table><tr><td colspan='2'>[[3b29]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3B29 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3B29 FirstGlance]. <br>
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==Disease==
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GSH:GLUTATHIONE'>GSH</scene>, <scene name='pdbligand=LSM:DODECYL+4-O-ALPHA-D-GLUCOPYRANOSYL-1-SELENO-BETA-D-GLUCOPYRANOSIDE'>LSM</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2pno|2pno]], [[3pcv|3pcv]]</td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">LTC4S ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])</td></tr>
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<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Leukotriene-C(4)_synthase Leukotriene-C(4) synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.4.1.20 4.4.1.20] </span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3b29 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3b29 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3b29 RCSB], [http://www.ebi.ac.uk/pdbsum/3b29 PDBsum]</span></td></tr>
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</table>
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== Disease ==
[[http://www.uniprot.org/uniprot/LTC4S_HUMAN LTC4S_HUMAN]] Defects in LTC4S are the cause of leukotriene C4 synthase deficiency (LTC4 synthase deficiency) [MIM:[http://omim.org/entry/246530 246530]]. LTC4 synthase deficiency is a fatal neurometabolic developmental disorder. It is associated with muscular hypotonia, psychomotor retardation, failure to thrive, and microcephaly.
[[http://www.uniprot.org/uniprot/LTC4S_HUMAN LTC4S_HUMAN]] Defects in LTC4S are the cause of leukotriene C4 synthase deficiency (LTC4 synthase deficiency) [MIM:[http://omim.org/entry/246530 246530]]. LTC4 synthase deficiency is a fatal neurometabolic developmental disorder. It is associated with muscular hypotonia, psychomotor retardation, failure to thrive, and microcephaly.
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== Function ==
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==Function==
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[[http://www.uniprot.org/uniprot/LTC4S_HUMAN LTC4S_HUMAN]] Catalyzes the conjugation of leukotriene A4 with reduced glutathione to form leukotriene C4.
[[http://www.uniprot.org/uniprot/LTC4S_HUMAN LTC4S_HUMAN]] Catalyzes the conjugation of leukotriene A4 with reduced glutathione to form leukotriene C4.
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Dodecyl-beta-D-selenomaltoside (SeDDM) is a seleno-detergent with a beta-glycosidic seleno-ether in place of the ether moiety in dodecyl-beta-D-maltoside. Seleno-detergents are candidates for heavy-atom agents in experimental phasing of membrane proteins in protein crystallography. Crystals of a nuclear membrane-embedded enzyme, leukotriene C(4) synthase (LTC(4)S), in complex with SeDDM were prepared and a multiwavelength anomalous diffraction (MAD) experiment was performed. The SeDDM in the LTC(4)S crystal exhibited sufficient anomalous diffraction for determination of the structure using MAD phasing.
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==About this Structure==
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Seleno-detergent MAD phasing of leukotriene C4 synthase in complex with dodecyl-beta-D-selenomaltoside.,Saino H, Ago H, Ukita Y, Miyano M Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 Dec 1;67(Pt 12):1666-73., Epub 2011 Nov 29. PMID:22139193<ref>PMID:22139193</ref>
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[[3b29]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3B29 OCA].
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
==See Also==
==See Also==
*[[Leukotriene C4 synthase|Leukotriene C4 synthase]]
*[[Leukotriene C4 synthase|Leukotriene C4 synthase]]
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== References ==
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==Reference==
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<references/>
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<ref group="xtra">PMID:022139193</ref><references group="xtra"/><references/>
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__TOC__
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</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Ago, H.]]
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[[Category: Ago, H]]
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[[Category: Miyano, M.]]
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[[Category: Miyano, M]]
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[[Category: Saino, H.]]
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[[Category: Saino, H]]
[[Category: Helix bundle]]
[[Category: Helix bundle]]
[[Category: Homo trimer]]
[[Category: Homo trimer]]

Revision as of 13:10, 18 December 2014

Human leukotriene C4 synthase in complex with dodecyl-beta-D-selenomaltoside

3b29, resolution 3.20Å

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