2ymz
From Proteopedia
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| - | + | ==Crystal structure of chicken Galectin 2== | |
| - | === | + | <StructureSection load='2ymz' size='340' side='right' caption='[[2ymz]], [[Resolution|resolution]] 1.75Å' scene=''> |
| - | + | == Structural highlights == | |
| + | <table><tr><td colspan='2'>[[2ymz]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2YMZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2YMZ FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=LAT:BETA-LACTOSE'>LAT</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ymz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ymz OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2ymz RCSB], [http://www.ebi.ac.uk/pdbsum/2ymz PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The comparatively small number of members of the family of adhesion/growth-regulatory galectins in chicken predestines this system as an attractive model to study the divergence of these lectins after gene duplication. Expression profiling of the three homodimeric (prototype) chicken galectins (CG-1A, CG-1B and CG-2) has raised evidence of distinct functionalities, explaining the interest in a detailed crystallographic analysis of CG-2. As revealed here, marked differences are found in the ligand-binding site and in the contact pattern within the homodimer interface, underlying a characteristic orientation of the two subunits. Notably, a distinctive trimer of dimers that is unique in all galectin crystal structures reported to date forms the core unit of the crystallographic assembly. Combination with spectroscopic and thermodynamic measurements, and comparisons with CG-1A and CG-1B, identify differential changes in the circular-dichroism spectra in the presence of lactose, reflecting the far-reaching impact of the ligand on hydrodynamic behaviour, and inter-galectin differences in both the entropy and the enthalpy of binding. This structural information is a salient step to complete the analysis of the full set of galectins from this model organism. | ||
| - | + | Fine-tuning of prototype chicken galectins: structure of CG-2 and structure-activity correlations.,Ruiz FM, Fernandez IS, Lopez-Merino L, Lagartera L, Kaltner H, Menendez M, Andre S, Solis D, Gabius HJ, Romero A Acta Crystallogr D Biol Crystallogr. 2013 Sep 1;69(Pt 9):1665-76. doi:, 10.1107/S0907444913011773. Epub 2013 Aug 15. PMID:23999290<ref>PMID:23999290</ref> | |
| - | + | ||
| - | == | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> |
| - | + | </div> | |
| + | |||
| + | ==See Also== | ||
| + | *[[Galectin|Galectin]] | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
[[Category: Gallus gallus]] | [[Category: Gallus gallus]] | ||
| - | [[Category: Fernandez, I S | + | [[Category: Fernandez, I S]] |
| - | [[Category: Gabius, H J | + | [[Category: Gabius, H J]] |
| - | [[Category: Romero, A | + | [[Category: Romero, A]] |
| - | [[Category: Ruiz, F M | + | [[Category: Ruiz, F M]] |
| - | [[Category: Solis, D | + | [[Category: Solis, D]] |
[[Category: Carbohydrate recognition]] | [[Category: Carbohydrate recognition]] | ||
[[Category: Sugar binding protein]] | [[Category: Sugar binding protein]] | ||
Revision as of 13:11, 18 December 2014
Crystal structure of chicken Galectin 2
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