3gfi
From Proteopedia
(Difference between revisions)
| Line 1: | Line 1: | ||
| - | + | ==Crystal structure of ST1710 complexed with its promoter DNA== | |
| - | === | + | <StructureSection load='3gfi' size='340' side='right' caption='[[3gfi]], [[Resolution|resolution]] 2.10Å' scene=''> |
| - | + | == Structural highlights == | |
| + | <table><tr><td colspan='2'>[[3gfi]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Sulfolobus_tokodaii Sulfolobus tokodaii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3GFI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3GFI FirstGlance]. <br> | ||
| + | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2eb7|2eb7]], [[2yr2|2yr2]], [[3gez|3gez]], [[3gf2|3gf2]], [[3gfj|3gfj]], [[3gfl|3gfl]], [[3gfm|3gfm]]</td></tr> | ||
| + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">ST1710 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=111955 Sulfolobus tokodaii])</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3gfi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3gfi OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3gfi RCSB], [http://www.ebi.ac.uk/pdbsum/3gfi PDBsum], [http://www.topsan.org/Proteins/RSGI/3gfi TOPSAN]</span></td></tr> | ||
| + | </table> | ||
| + | == Evolutionary Conservation == | ||
| + | [[Image:Consurf_key_small.gif|200px|right]] | ||
| + | Check<jmol> | ||
| + | <jmolCheckbox> | ||
| + | <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/gf/3gfi_consurf.spt"</scriptWhenChecked> | ||
| + | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | ||
| + | <text>to colour the structure by Evolutionary Conservation</text> | ||
| + | </jmolCheckbox> | ||
| + | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf]. | ||
| + | <div style="clear:both"></div> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | ST1710, a member of the multiple antibiotic resistance regulator (MarR) family of regulatory proteins in bacteria and archaea, plays important roles in development of antibiotic resistance, a global health problem. Here, we present the crystal structure of ST1710 from Sulfolobus tokodaii strain 7 complexed with salicylate, a well-known inhibitor of MarR proteins and the ST1710 complex with its promoter DNA, refined to 1.8 and 2.10 A resolutions, respectively. The ST1710-DNA complex shares the topology of apo-ST1710 and MarR proteins, with each subunit containing a winged helix-turn-helix (wHtH) DNA binding motif. Significantly large conformational changes occurred upon DNA binding and in each of the dimeric monomers in the asymmetric unit of the ST1710-DNA complex. Conserved wHtH loop residues interacting with the bound DNA and mutagenic analysis indicated that R89, R90 and K91 were important for DNA recognition. Significantly, the bound DNA exhibited a new binding mechanism. | ||
| - | + | ST1710-DNA complex crystal structure reveals the DNA binding mechanism of the MarR family of regulators.,Kumarevel T, Tanaka T, Umehara T, Yokoyama S Nucleic Acids Res. 2009 Aug;37(14):4723-35. Epub 2009 Jun 9. PMID:19509310<ref>PMID:19509310</ref> | |
| - | + | ||
| - | == | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> |
| - | + | </div> | |
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
[[Category: Sulfolobus tokodaii]] | [[Category: Sulfolobus tokodaii]] | ||
| - | [[Category: Kumarevel, T | + | [[Category: Kumarevel, T]] |
| - | [[Category: Tanaka, T | + | [[Category: Tanaka, T]] |
| - | [[Category: Yokoyama, S | + | [[Category: Yokoyama, S]] |
[[Category: Dna-binding]] | [[Category: Dna-binding]] | ||
[[Category: Marr]] | [[Category: Marr]] | ||
[[Category: National project on protein structural and functional analyse]] | [[Category: National project on protein structural and functional analyse]] | ||
[[Category: Nppsfa]] | [[Category: Nppsfa]] | ||
| - | [[Category: | + | [[Category: Structural genomic]] |
[[Category: Rsgi]] | [[Category: Rsgi]] | ||
[[Category: St1710]] | [[Category: St1710]] | ||
| - | [[Category: Structural genomic]] | ||
[[Category: Transcription]] | [[Category: Transcription]] | ||
[[Category: Transcription regulation]] | [[Category: Transcription regulation]] | ||
[[Category: Transcription regulator]] | [[Category: Transcription regulator]] | ||
[[Category: Transcription-dna complex]] | [[Category: Transcription-dna complex]] | ||
Revision as of 13:17, 18 December 2014
Crystal structure of ST1710 complexed with its promoter DNA
| |||||||||||
Categories: Sulfolobus tokodaii | Kumarevel, T | Tanaka, T | Yokoyama, S | Dna-binding | Marr | National project on protein structural and functional analyse | Nppsfa | Structural genomic | Rsgi | St1710 | Transcription | Transcription regulation | Transcription regulator | Transcription-dna complex
