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3ahu
From Proteopedia
(Difference between revisions)
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| - | + | ==Crystal structure of YmaH (Hfq) from Bacillus subtilis in complex with an RNA aptamer.== | |
| - | + | <StructureSection load='3ahu' size='340' side='right' caption='[[3ahu]], [[Resolution|resolution]] 2.20Å' scene=''> | |
| - | + | == Structural highlights == | |
| + | <table><tr><td colspan='2'>[[3ahu]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3AHU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3AHU FirstGlance]. <br> | ||
| + | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">BSU17340, hfq, ymaH ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1423 Bacillus subtilis])</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ahu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ahu OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3ahu RCSB], [http://www.ebi.ac.uk/pdbsum/3ahu PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The Hfq protein is a hexameric RNA-binding protein which regulates gene expression by binding to RNA under the influence of diverse environmental stresses. Its ring structure binds various types of RNA, including mRNA and sRNA. RNA-bound structures of Hfq from Escherichia coli and Staphylococcus aureus have been revealed to have poly(A) RNA at the distal site and U-rich RNA at the proximal site, respectively. Here, crystals of a complex of the Bacillus subtilis Hfq protein with an A/G-repeat 7-mer RNA (Hfq-RNA) that were prepared using the hanging-drop vapour-diffusion technique are reported. The type 1 Hfq-RNA crystals belonged to space group I422, with unit-cell parameters a = b = 123.70, c = 119.13 A, while the type 2 Hfq-RNA crystals belonged to space group F222, with unit-cell parameters a = 91.92, b = 92.50, c = 114.92 A. Diffraction data were collected to a resolution of 2.20 A from both crystal forms. The hexameric structure of the Hfq protein was clearly shown by self-rotation analysis. | ||
| - | + | Expression, crystallization and preliminary crystallographic analysis of RNA-binding protein Hfq (YmaH) from Bacillus subtilis in complex with an RNA aptamer.,Baba S, Someya T, Kawai G, Nakamura K, Kumasaka T Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 May 1;66(Pt, 5):563-6. Epub 2010 Apr 30. PMID:20445260<ref>PMID:20445260</ref> | |
| - | + | ||
| - | == | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> |
| - | + | </div> | |
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
[[Category: Bacillus subtilis]] | [[Category: Bacillus subtilis]] | ||
| - | [[Category: Baba, S | + | [[Category: Baba, S]] |
| - | [[Category: Kawai, G | + | [[Category: Kawai, G]] |
| - | [[Category: Kumasaka, T | + | [[Category: Kumasaka, T]] |
| - | [[Category: Nakamura, K | + | [[Category: Nakamura, K]] |
| - | [[Category: Someya, T | + | [[Category: Someya, T]] |
[[Category: Protein-rna complex]] | [[Category: Protein-rna complex]] | ||
[[Category: Sm-like motif]] | [[Category: Sm-like motif]] | ||
[[Category: Translation-rna complex]] | [[Category: Translation-rna complex]] | ||
Revision as of 13:53, 18 December 2014
Crystal structure of YmaH (Hfq) from Bacillus subtilis in complex with an RNA aptamer.
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