2yc2
From Proteopedia
(Difference between revisions)
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| - | + | ==INTRAFLAGELLAR TRANSPORT COMPLEX 25-27 FROM CHLAMYDOMONAS== | |
| - | + | <StructureSection load='2yc2' size='340' side='right' caption='[[2yc2]], [[Resolution|resolution]] 2.59Å' scene=''> | |
| - | + | == Structural highlights == | |
| + | <table><tr><td colspan='2'>[[2yc2]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Chlamydomonas_reinhardtii Chlamydomonas reinhardtii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2YC2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2YC2 FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr> | ||
| + | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2yc4|2yc4]]</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2yc2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2yc2 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2yc2 RCSB], [http://www.ebi.ac.uk/pdbsum/2yc2 PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The cilium is an important organelle that is found on many eukaryotic cells, where it serves essential functions in motility, sensory reception and signalling. Intraflagellar transport (IFT) is a vital process for the formation and maintenance of cilia. We have determined the crystal structure of Chlamydomonas reinhardtii IFT25/27, an IFT sub-complex, at 2.6 A resolution. IFT25 and IFT27 interact via a conserved interface that we verify biochemically using structure-guided mutagenesis. IFT27 displays the fold of Rab-like small guanosine triphosphate hydrolases (GTPases), binds GTP and GDP with micromolar affinity and has very low intrinsic GTPase activity, suggesting that it likely requires a GTPase-activating protein (GAP) for robust GTP turnover. A patch of conserved surface residues contributed by both IFT25 and IFT27 is found adjacent to the GTP-binding site and could mediate the binding to other IFT proteins as well as to a potential GAP. These results provide the first step towards a high-resolution structural understanding of the IFT complex. | ||
| - | + | Crystal structure of the intraflagellar transport complex 25/27.,Bhogaraju S, Taschner M, Morawetz M, Basquin C, Lorentzen E EMBO J. 2011 Apr 19. PMID:21505417<ref>PMID:21505417</ref> | |
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| - | == | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> |
| - | + | </div> | |
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
[[Category: Chlamydomonas reinhardtii]] | [[Category: Chlamydomonas reinhardtii]] | ||
| - | [[Category: Bhogaraju, S | + | [[Category: Bhogaraju, S]] |
| - | [[Category: Lorentzen, E | + | [[Category: Lorentzen, E]] |
| - | [[Category: Taschner, M | + | [[Category: Taschner, M]] |
[[Category: Cilium]] | [[Category: Cilium]] | ||
[[Category: Ift complex]] | [[Category: Ift complex]] | ||
[[Category: Transport protein]] | [[Category: Transport protein]] | ||
Revision as of 14:19, 18 December 2014
INTRAFLAGELLAR TRANSPORT COMPLEX 25-27 FROM CHLAMYDOMONAS
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