This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
2yc2
From Proteopedia
(Difference between revisions)
| Line 1: | Line 1: | ||
| - | + | ==INTRAFLAGELLAR TRANSPORT COMPLEX 25-27 FROM CHLAMYDOMONAS== | |
| - | + | <StructureSection load='2yc2' size='340' side='right' caption='[[2yc2]], [[Resolution|resolution]] 2.59Å' scene=''> | |
| - | + | == Structural highlights == | |
| + | <table><tr><td colspan='2'>[[2yc2]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Chlamydomonas_reinhardtii Chlamydomonas reinhardtii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2YC2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2YC2 FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr> | ||
| + | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2yc4|2yc4]]</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2yc2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2yc2 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2yc2 RCSB], [http://www.ebi.ac.uk/pdbsum/2yc2 PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The cilium is an important organelle that is found on many eukaryotic cells, where it serves essential functions in motility, sensory reception and signalling. Intraflagellar transport (IFT) is a vital process for the formation and maintenance of cilia. We have determined the crystal structure of Chlamydomonas reinhardtii IFT25/27, an IFT sub-complex, at 2.6 A resolution. IFT25 and IFT27 interact via a conserved interface that we verify biochemically using structure-guided mutagenesis. IFT27 displays the fold of Rab-like small guanosine triphosphate hydrolases (GTPases), binds GTP and GDP with micromolar affinity and has very low intrinsic GTPase activity, suggesting that it likely requires a GTPase-activating protein (GAP) for robust GTP turnover. A patch of conserved surface residues contributed by both IFT25 and IFT27 is found adjacent to the GTP-binding site and could mediate the binding to other IFT proteins as well as to a potential GAP. These results provide the first step towards a high-resolution structural understanding of the IFT complex. | ||
| - | + | Crystal structure of the intraflagellar transport complex 25/27.,Bhogaraju S, Taschner M, Morawetz M, Basquin C, Lorentzen E EMBO J. 2011 Apr 19. PMID:21505417<ref>PMID:21505417</ref> | |
| - | + | ||
| - | == | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> |
| - | + | </div> | |
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
[[Category: Chlamydomonas reinhardtii]] | [[Category: Chlamydomonas reinhardtii]] | ||
| - | [[Category: Bhogaraju, S | + | [[Category: Bhogaraju, S]] |
| - | [[Category: Lorentzen, E | + | [[Category: Lorentzen, E]] |
| - | [[Category: Taschner, M | + | [[Category: Taschner, M]] |
[[Category: Cilium]] | [[Category: Cilium]] | ||
[[Category: Ift complex]] | [[Category: Ift complex]] | ||
[[Category: Transport protein]] | [[Category: Transport protein]] | ||
Revision as of 14:19, 18 December 2014
INTRAFLAGELLAR TRANSPORT COMPLEX 25-27 FROM CHLAMYDOMONAS
| |||||||||||
