1q5z
From Proteopedia
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| - | [[Image:1q5z.jpg|left|200px]] | + | [[Image:1q5z.jpg|left|200px]] |
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| - | '''Crystal Structure of the C-terminal Actin Binding Domain of Salmonella Invasion Protein A (SipA)''' | + | {{Structure |
| + | |PDB= 1q5z |SIZE=350|CAPTION= <scene name='initialview01'>1q5z</scene>, resolution 1.8Å | ||
| + | |SITE= | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= | ||
| + | |GENE= SipA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=602 Salmonella typhimurium]) | ||
| + | }} | ||
| + | |||
| + | '''Crystal Structure of the C-terminal Actin Binding Domain of Salmonella Invasion Protein A (SipA)''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1Q5Z is a [ | + | 1Q5Z is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Salmonella_typhimurium Salmonella typhimurium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Q5Z OCA]. |
==Reference== | ==Reference== | ||
| - | Salmonella SipA polymerizes actin by stapling filaments with nonglobular protein arms., Lilic M, Galkin VE, Orlova A, VanLoock MS, Egelman EH, Stebbins CE, Science. 2003 Sep 26;301(5641):1918-21. PMID:[http:// | + | Salmonella SipA polymerizes actin by stapling filaments with nonglobular protein arms., Lilic M, Galkin VE, Orlova A, VanLoock MS, Egelman EH, Stebbins CE, Science. 2003 Sep 26;301(5641):1918-21. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/14512630 14512630] |
[[Category: Salmonella typhimurium]] | [[Category: Salmonella typhimurium]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: cell invasion]] | [[Category: cell invasion]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 13:33:23 2008'' |
Revision as of 11:33, 20 March 2008
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| , resolution 1.8Å | |||||||
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| Gene: | SipA (Salmonella typhimurium) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal Structure of the C-terminal Actin Binding Domain of Salmonella Invasion Protein A (SipA)
Overview
Like many bacterial pathogens, Salmonella spp. use a type III secretion system to inject virulence proteins into host cells. The Salmonella invasion protein A (SipA) binds host actin, enhances its polymerization near adherent extracellular bacteria, and contributes to cytoskeletal rearrangements that internalize the pathogen. By combining x-ray crystallography of SipA with electron microscopy and image analysis of SipA-actin filaments, we show that SipA functions as a "molecular staple," in which a globular domain and two nonglobular "arms" mechanically stabilize the filament by tethering actin subunits in opposing strands. Deletion analysis of the tethering arms provides strong support for this model.
About this Structure
1Q5Z is a Single protein structure of sequence from Salmonella typhimurium. Full crystallographic information is available from OCA.
Reference
Salmonella SipA polymerizes actin by stapling filaments with nonglobular protein arms., Lilic M, Galkin VE, Orlova A, VanLoock MS, Egelman EH, Stebbins CE, Science. 2003 Sep 26;301(5641):1918-21. PMID:14512630
Page seeded by OCA on Thu Mar 20 13:33:23 2008
